Alfaro A, Catelain C, El-Masri H, Rameau P, Lacroix-Triki M, Scoazec J Y, Marty V, Mosele F, Pistilli B
Gustave Roussy, UMS AMMICa, CNRS UAR 3655, INSERM US23, 114 rue Edouard Vaillant, Villejuif, F-94805, France.
Gustave Roussy, Department of Medical Oncology- Breast Cancer Unit U981, 114 rue Edouard Vaillant, Villejuif, F-94805, France.
NPJ Breast Cancer. 2024 Sep 14;10(1):81. doi: 10.1038/s41523-024-00691-x.
Medullary carcinoma of the breast (MedBC) is a rare histological type that accounts for less than 5% of all invasive breast cancers. Here, we performed an exploratory study aimed to determine whether imaging mass cytometry (IMC) can be used to characterize the immune infiltration and the spatial distribution heterogeneity in the rare subtype of MedBC compared to atypical MedBC and TNBC-TILS tumors. In both MedBC and TNBC-TILs, there was a notable enrichment of immune cells in the peripheral regions of the tumors, whereas in atypical MedBC, the immune cells exhibited a central enrichment pattern. This distribution of infiltrated cells reflects an active immune recruitment correlated to more favorable prognosis. In MedBC, spatial analysis shows that immune cells are localized at a greater distance from the tumor cells. IMC highlights the heterogeneity of immune microenvironment across three main subtypes of breast tumors and could help to define distinct immune patterns.
乳腺髓样癌（MedBC）是一种罕见的组织学类型，占所有浸润性乳腺癌的比例不到5%。在此，我们进行了一项探索性研究，旨在确定与非典型MedBC和三阴性乳腺癌肿瘤浸润淋巴细胞（TNBC-TILS）肿瘤相比，成像质谱流式细胞术（IMC）是否可用于表征这种罕见的MedBC亚型中的免疫浸润和空间分布异质性。在MedBC和TNBC-TILs中，肿瘤周边区域的免疫细胞均显著富集，而在非典型MedBC中，免疫细胞呈现中央富集模式。浸润细胞的这种分布反映了与更有利预后相关的活跃免疫募集。在MedBC中，空间分析表明免疫细胞定位在距肿瘤细胞更远的位置。IMC突出了乳腺肿瘤三种主要亚型中免疫微环境的异质性，并有助于定义不同的免疫模式。
