Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing, 400712, PR China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture and Rural Affairs, Citrus Research Institute, Southwest University, Chongqing, 400712, PR China; National Citrus Engineering Research Center, Chongqing, 400712, PR China.
Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing, 400712, PR China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture and Rural Affairs, Citrus Research Institute, Southwest University, Chongqing, 400712, PR China; National Citrus Engineering Research Center, Chongqing, 400712, PR China.
Talanta. 2025 Jan 1;281:126891. doi: 10.1016/j.talanta.2024.126891. Epub 2024 Sep 14.
The wide use and high toxicity of carbendazim (CBD) in agriculture pose unprecedented demands for convenient, sensitive, and cost-effective on-site monitoring. Herein, we propose a novel colorimetric and photothermal dual-mode lateral flow immunoassay (LFIA) based on plasmonic gold nanostars (AuNSs) for CBD detection in agricultural products. The AuNSs were synthesized via a rapid seed-mediated growth method (with growth time of ∼5 s). A stable immunoprobe was formed by adsorbing CBD antibodies onto AuNSs. This immunoprobe exhibited high conversion efficiency and sensitivity in photothermal detection with a low limit of detection (LOD) of 0.28 ng mL. The LOD of the colorimetric mode was higher (0.48 ng mL). The results of CBD detection in various agricultural products aligned well with ultra-performance liquid chromatography tandem mass spectrometry. Overall, our LFIA shows excellent sensitivity, specificity, reproducibility, and rapidness in CBD detection, and thus is a highly potential on-site platform in resource-limited environments.
多菌灵（CBD）在农业中的广泛使用和高毒性对方便、敏感和具有成本效益的现场监测提出了前所未有的要求。在此，我们提出了一种基于等离子体金纳米星（AuNSs）的新型比色和光热双模式侧向流动免疫分析（LFIA），用于检测农产品中的 CBD。AuNSs 通过快速种子介导的生长方法（生长时间约为 5 秒）合成。CBD 抗体吸附在 AuNSs 上形成稳定的免疫探针。该免疫探针在光热检测中表现出高转换效率和灵敏度，检测限（LOD）低至 0.28ng/mL。比色模式的 LOD 更高（0.48ng/mL）。各种农产品中 CBD 的检测结果与超高效液相色谱串联质谱法吻合较好。总的来说，我们的 LFIA 在 CBD 检测中表现出优异的灵敏度、特异性、重现性和快速性，因此是资源有限环境中极具潜力的现场平台。
