Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, China.
Anal Bioanal Chem. 2021 Jun;413(14):3725-3735. doi: 10.1007/s00216-021-03321-8. Epub 2021 Apr 13.
In this study, for the first time, we propose a sensitive colloidal gold-based lateral flow immunoassay (LFIA) that can be used to detect carbendazim residues in functional foods. The adoption of inline cleanup LFIA strips effectively improved background interference to reduce misjudgment of results. First, the hapten 2-(methylamino)-1H-benzo[d]imidazole-5-carboxylic acid was used to establish the carbendazim immunoassay method. Subsequently, colloidal gold-mAb preparation and LFIA detection conditions were systematically optimized. For root and fruit samples (ginseng, ginger, jujube, and Chinese wolfberry), the designed strips had a cutoff value of 8 ng/mL. For flower and seed samples (chrysanthemum, coix seed, and malt), the cutoff value was 12 ng/mL. Even in a complex matrix, the established LFIA method demonstrates satisfactory sensitivity and anti-interference ability. This method was successfully applied in detection of carbendazim residues in complex functional foods, and the assay results are consistent with those obtained via liquid chromatography-tandem mass spectrometry. In short, the proposed method is fast and sensitive and has strong anti-interference ability. Furthermore, it provides a new technical method highly relevant to the on-site rapid detection of carbendazim residues in complex sample matrix.
在这项研究中，我们首次提出了一种敏感的基于胶体金的侧向流动免疫分析（LFIA）方法，可用于检测功能性食品中的多菌灵残留。采用在线净化 LFIA 条带可有效改善背景干扰，减少结果误判。首先，使用半抗原 2-（甲氨基）-1H-苯并[d]咪唑-5-羧酸建立了多菌灵免疫分析方法。随后，系统优化了胶体金-mAb 制备和 LFIA 检测条件。对于根和果实样品（人参、生姜、红枣和枸杞），设计的条带的截止值为 8ng/mL。对于花和种子样品（菊花、薏苡仁和麦芽），截止值为 12ng/mL。即使在复杂的基质中，所建立的 LFIA 方法也表现出令人满意的灵敏度和抗干扰能力。该方法已成功应用于复杂功能性食品中多菌灵残留的检测，检测结果与液相色谱-串联质谱法一致。总之，该方法快速、灵敏，具有较强的抗干扰能力。此外，它为现场快速检测复杂样品基质中的多菌灵残留提供了一种新的相关技术方法。
