Department of Neurobiology, University of California San Diego, United States.
Department of Neurology, University of Texas Southwestern Medical Center, United States.
J Neuroimmunol. 2024 Nov 15;396:578454. doi: 10.1016/j.jneuroim.2024.578454. Epub 2024 Sep 8.
Autoimmune autonomic ganglionopathy (AAG) is a rare disease wherein autoantibodies target the ganglionic acetylcholine receptor (gAChR). Current diagnosis in the United States depends upon clinical symptoms and positive autoantibody detection using a radioimmunoprecipitation assay (RIA). Here we offer a proof-of-principle study on an alternative method, fluorescence-detection size-exclusion-chromatography (FSEC). We show FSEC can detect autoantibodies against gAChR from patient sera but not healthy controls or samples from other autoimmune diseases. We compare FSEC to RIA and find good correlation. We discuss potential advantages of using FSEC as an alternative or as a first-step diagnostic prior to pursuing existing methodologies.
自身免疫性自主神经节病（AAG）是一种罕见的疾病，自身抗体靶向 ganglionic 乙酰胆碱受体（gAChR）。目前，美国的诊断依赖于临床症状和使用放射免疫沉淀测定（RIA）进行的自身抗体阳性检测。在这里，我们提供了一种替代方法——荧光检测排阻色谱法（FSEC）的原理验证研究。我们表明，FSEC 可以检测来自患者血清的针对 gAChR 的自身抗体，但不能检测健康对照或来自其他自身免疫性疾病的样本。我们将 FSEC 与 RIA 进行比较，发现相关性良好。我们讨论了使用 FSEC 作为替代方法或在采用现有方法之前作为第一步诊断的潜在优势。
