Department of Ophthalmology and Visual Science, W.K. Kellogg Eye Center, University of Michigan, Ann Arbor, MI, 48105, USA.
Department of Ophthalmology and Visual Science, W.K. Kellogg Eye Center, University of Michigan, Ann Arbor, MI, 48105, USA; Department of Pathology, University of Michigan, Ann Arbor, MI, 48105, USA.
Biochem Biophys Res Commun. 2024 Nov 12;733:150668. doi: 10.1016/j.bbrc.2024.150668. Epub 2024 Sep 10.
Regulation of gene expression is achieved through the modulation of regulatory inputs both pre- and post-transcriptionally. Methyltransferase-like 3 (METTL3) is a key player in pre-mRNA processing, actively catalyzing N6-methyladenosine (m6A). Among the most enriched mRNA targets of METTL3 is the Ras Responsive Element Binding Protein 1 (RREB1), a transcription factor which functions to govern cell fate, proliferation and DNA repair. Here, we show a novel interaction between METTL3 and RREB1. Further examination of this interaction indicates that METTL3's N-terminus is the primary interacting domain. Our findings uncover a novel interacting partner of METTL3, providing further insights into METTL3's regulatory network.
基因表达的调控是通过转录前和转录后调节输入来实现的。甲基转移酶样 3（METTL3）是 mRNA 前体处理的关键因子，积极催化 N6-甲基腺苷（m6A）。METTL3 最富集的 mRNA 靶标之一是 Ras 反应元件结合蛋白 1（RREB1），它是一种转录因子，用于控制细胞命运、增殖和 DNA 修复。在这里，我们展示了 METTL3 和 RREB1 之间的一种新的相互作用。对这种相互作用的进一步研究表明，METTL3 的 N 端是主要的相互作用域。我们的发现揭示了 METTL3 的一个新的相互作用伙伴，为 METTL3 的调控网络提供了进一步的见解。
