Alvizo-Rodríguez Carlos Rogelio, Calzada Fernando, López-Vázquez Uriel, Tiburcio Emmanuel Tomay, Hernandez-Rivera Juan A, Carrasco-Carballo Alan, Hernández-Caballero Marta Elena
Facultad de Medicina, Biomedicina, Benemérita Universidad Autónoma de Puebla, 13 Sur 2702 Col. Volcanes, Puebla 72410, Mexico.
Unidad de Investigación Médica en Farmacología, Unidad Médica de Alta Especialidad, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, Av. Cuauhtémoc 330, Colonia Doctores, Mexico City 06720, Mexico.
Molecules. 2025 Jun 18;30(12):2634. doi: 10.3390/molecules30122634.
is used in traditional medicine to treat cancer, as it contains flavonoids and phenols known to regulate key cellular processes associated with cancer. Breast cancer, the most common cancer among women globally, presents ongoing challenges in treatment. The discovery of mA methylation and its regulation by methylosome proteins offers novel therapeutic avenues for cancer management. This study aimed to investigate the cytotoxic and epitranscriptomic effects of an aqueous extract from on MCF-7 (luminal A) and HCC1937 (triple-negative) breast cancer cells. Cell lines were treated with the aqueous extract, characterized by HPLC, for 72 h, followed by an assessment of cytotoxicity and migration. The expression of methylosome components METTL3 and FTO was measured using RT-PCR. mA global methylation was assessed via colorimetry, and molecular docking studies were conducted. The results indicated that only HCC1937 cells exhibited altered migration capacity. This change was correlated in silico with the inhibition of METTL3 by luteolin and quercetin, constituents of the aqueous extract. METTL3, a methyltransferase, was overexpressed by scratch stimuli but was downregulated following treatment in both MCF-7 and HCC1937 cells. The FTO demethylase was overexpressed in both cell lines. In silico analysis suggested an interaction between FTO and compounds such as gallic acid and myricetin. Additionally, mA global methylation decreased in MCF-7 cells but increased in HCC1937 cells, potentially affecting cell migration. Our findings indicate that influences both METTL3 and FTO, altering mA methylation in a cell-type-dependent manner, with HCC1937 cells being particularly sensitive. Further research is required to elucidate the complete molecular mechanism of 's aqueous extract in breast cancer treatment.
传统医学中使用[提取物名称]来治疗癌症，因为它含有已知可调节与癌症相关的关键细胞过程的黄酮类化合物和酚类。乳腺癌是全球女性中最常见的癌症，在治疗方面面临持续挑战。甲基化修饰（mA methylation）及其由甲基osome蛋白调控的发现为癌症管理提供了新的治疗途径。本研究旨在调查[提取物名称]水提取物对MCF - 7（腔面A型）和HCC1937（三阴性）乳腺癌细胞的细胞毒性和表转录组学效应。细胞系用经高效液相色谱（HPLC）表征的[提取物名称]水提取物处理72小时，随后评估细胞毒性和迁移情况。使用逆转录聚合酶链反应（RT - PCR）测量甲基osome成分METTL3和FTO的表达。通过比色法评估mA整体甲基化，并进行分子对接研究。结果表明，只有HCC1937细胞表现出迁移能力改变。这种变化在计算机模拟中与水提取物成分木犀草素和槲皮素对METTL3的抑制相关。甲基转移酶METTL3在划痕刺激下过表达，但在MCF - 7和HCC1937细胞中经[提取物名称]处理后下调。FTO去甲基化酶在两种细胞系中均过表达。计算机模拟分析表明FTO与没食子酸和杨梅素等化合物之间存在相互作用。此外，MCF - 7细胞中mA整体甲基化降低，而HCC1937细胞中升高，这可能影响细胞迁移。我们的研究结果表明，[提取物名称]影响METTL3和FTO，以细胞类型依赖的方式改变mA甲基化，HCC1937细胞尤其敏感。需要进一步研究以阐明[提取物名称]水提取物在乳腺癌治疗中的完整分子机制。
