College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
Plant Sci. 2024 Dec;349:112266. doi: 10.1016/j.plantsci.2024.112266. Epub 2024 Sep 13.
Paeonia ostii var. lishizhenii exhibits superiority of high α-linolenic acid in seed oils, yet, the low yield highlights the importance of enhancing oil accumulation in seeds for edible oil production. The transcription factor protein WRINKLED1 (WRI1) plays crucial roles in modulating oil content in higher plants; however, its functional characterization remains elusive in P. ostii var. lishizhenii. Herein, based on a correlation analysis of transcription factor transcript levels, FA accumulation rates, and interaction assay of FA biosynthesis associated proteins, a WRI1 homologous gene (PoWRI1) that potentially regulated oil content in P. ostii var. lishizhenii seeds was screened. The PoWRI1 exhibited an endosperm-specific and development-depended expression pattern, encoding a nuclear-localized protein with transcriptional activation capability. Notably, overexpressing PoWRI1 upregulated certain key genes relevant to glycolysis, FA biosynthesis and desaturation, and improved seed development, oil body formation and oil accumulation in Arabidopsis seeds, resulting an enhancement of total seed oil weight by 9.47-18.77 %. The defective impacts on seed phenotypes were rescued through ectopic induction of PoWRI1 in wri1 mutants. Our findings highlight the pivotal role of PoWRI1 in controlling oil accumulation in P. ostii var. lishizhenii, offering bioengineering strategies to increase seed oil accumulation and enhance its potential for edible oil production.
牡丹芍药变种具有高α-亚麻酸含量的优势，但其种子产油量低，突出了提高食用植物油产量的重要性。转录因子蛋白 WRINKLED1（WRI1）在调节高等植物油含量方面起着关键作用；然而，其在牡丹芍药变种中的功能特征仍然难以捉摸。在此，基于转录因子转录水平、FA 积累率的相关分析和 FA 生物合成相关蛋白的相互作用测定，筛选出一个可能调节牡丹芍药变种种子油含量的 WRI1 同源基因（PoWRI1）。PoWRI1 表现出胚乳特异性和发育依赖性表达模式，编码一种具有转录激活能力的核定位蛋白。值得注意的是，过表达 PoWRI1 上调了与糖酵解、FA 生物合成和去饱和相关的某些关键基因，改善了拟南芥种子的发育、油体形成和油积累，使总种子油重增加了 9.47-18.77%。通过异位诱导 wri1 突变体中的 PoWRI1，挽救了对种子表型的缺陷影响。我们的研究结果强调了 PoWRI1 在控制牡丹芍药变种油积累中的关键作用，为提高种子油积累和增强其食用植物油生产潜力提供了生物工程策略。
