College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, Henan, China.
Henan Key Laboratory of Crop Molecular Breeding and Bioreactor, Zhoukou, 466001, Henan, China.
Genes Genomics. 2021 Aug;43(8):885-896. doi: 10.1007/s13258-021-01102-2. Epub 2021 Apr 21.
Paeonia ostii seeds were identified as novel sources of edible plant oil with a high proportion of α-linolenic acid, a type of n-3 fatty acid with many health benefits. Due to the unreliability of seed oil content and quality, it is necessary to discover the mechanism underlying lipid biosynthesis in Paeonia ostii seeds.
This study aimed to identify the key genes involved in lipid biosynthesis in Paeonia ostii seeds by analyzing the relationship among the seed characteristics and the expression patterns of lipid genes in Paeonia ostii during seed development.
Preliminary research on Paeonia ostii seed development was carried out from 10 days after pollination until maturity, focusing on phenology, oil content and lipid profiles. In addition, we investigated the spatiotemporal expression of 36 lipid biosynthetic genes in Paeonia ostii by using quantitative real-time PCR.
The results suggested that the development of Paeonia ostii seeds from pollination to maturity could be divided into three periods. The 36 lipid genes showed various spatiotemporal expression patterns and five gene groups with distinct temporal patterns during seed development were identified by clustering analysis of expression data. Furthermore, the relationships between gene expression and lipid/fatty acid accumulation and some candidate key lipid genes were discussed.
This study provided the global patterns of fatty acid and lipid biosynthesis-related gene expression, which are critical to understanding the molecular basis of lipid biosynthesis and identifying the lipid accumulation rate-limiting genes during seed development.
牡丹籽油是一种新型的食用植物油，其 α-亚麻酸含量较高，α-亚麻酸是一种 n-3 脂肪酸，具有许多健康益处。由于种子含油率和质量不稳定，因此有必要发现牡丹籽油生物合成的机制。
本研究旨在通过分析牡丹籽油种子发育过程中种子特性与脂类基因表达模式之间的关系，鉴定牡丹籽油种子脂类生物合成中的关键基因。
对授粉后 10 天至成熟的牡丹籽油种子发育进行初步研究，重点研究其物候学、含油量和脂类谱。此外，我们还通过定量实时 PCR 研究了 36 个脂类生物合成基因在牡丹籽油种子发育过程中的时空表达。
结果表明，从授粉到成熟，牡丹籽油种子的发育可以分为三个时期。36 个脂类基因表现出不同的时空表达模式，通过对表达数据进行聚类分析，共鉴定出 5 个具有明显时间模式的基因簇。此外，还讨论了基因表达与脂类/脂肪酸积累之间的关系，以及一些候选关键脂类基因。
本研究提供了脂肪酸和脂类生物合成相关基因表达的全局模式，这对理解脂类生物合成的分子基础以及鉴定种子发育过程中的脂类积累限速基因具有重要意义。
