Department of Radiation Oncology, Mayo Clinic, 4450 San Pablo Rd S, Jacksonville, FL 32224, USA.
J Radiat Res. 2024 Sep 24;65(5):651-657. doi: 10.1093/jrr/rrae071.
The repair of DNA double-strand breaks is a crucial yet delicate process which is affected by a multitude of factors. In this study, our goal is to analyse the influence of the linear energy transfer (LET) on the DNA repair kinetics. By utilizing the database of repair of DNA and aggregating the results of 84 experiments, we conduct various model fits to evaluate and compare different hypothesis regarding the effect of LET on the rejoining of DNA ends. Despite the considerable research efforts dedicated to this topic over the past decades, our findings underscore the complexity of the relationship between LET and DNA repair kinetics. This study leverages big data analysis to capture overall trends that single experimental studies might miss, providing a valuable model for understanding how radiation quality impacts DNA damage and subsequent biological effects. Our results highlight the gaps in our current understanding, emphasizing the pressing need for further investigation into this phenomenon.
DNA 双链断裂的修复是一个至关重要但又精细的过程，受到多种因素的影响。在这项研究中，我们的目标是分析线性能量转移（LET）对 DNA 修复动力学的影响。通过利用 DNA 修复数据库并汇总 84 项实验的结果，我们进行了各种模型拟合，以评估和比较关于 LET 对 DNA 末端重连影响的不同假设。尽管在过去几十年中，人们在这个主题上投入了相当大的研究努力，但我们的研究结果强调了 LET 和 DNA 修复动力学之间关系的复杂性。本研究利用大数据分析来捕捉单实验研究可能错过的总体趋势，为理解辐射质量如何影响 DNA 损伤和随后的生物学效应提供了一个有价值的模型。我们的研究结果突出了目前认识上的差距，强调了对这一现象进行进一步研究的迫切需要。
