Bengochea L, Ouviña G, Lemberg A
Biochem Biophys Res Commun. 1985 Jul 16;130(1):163-7. doi: 10.1016/0006-291x(85)90396-1.
The activity of bilirubin UDP-Glucuronyltransferase was determined in microsomes from normal and bile duct ligated rats. It was measured after 2 and 8 days following bile duct ligation and compared with normal rats. A decrease of 33% in the total enzyme activity was observed on day 2; a fall of 70% was founded on day 8. Bilirubin diglucuronide represented approximately 20% of total conjugates in both groups of cholestatic rats, as compared with 65% found in normals. It was concluded that bilirubin microsomal conjugating capacity is markedly altered during cholestasis. This can be attributed to microsomal membrane damage produced by stagnant bile.
在正常大鼠和胆管结扎大鼠的微粒体中测定了胆红素UDP-葡萄糖醛酸基转移酶的活性。在胆管结扎后2天和8天进行测量，并与正常大鼠进行比较。在第2天观察到总酶活性下降了33%；在第8天发现下降了70%。与正常大鼠中65%的比例相比，两组胆汁淤积大鼠中胆红素双葡萄糖醛酸酯约占总结合物的20%。得出的结论是，胆汁淤积期间胆红素微粒体结合能力明显改变。这可归因于停滞胆汁产生的微粒体膜损伤。
