Ouviña C B, Pavese C B, Lemberg A, Bengochea L A
Cátedra de Fisiopatología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Arch Physiol Biochem. 1995 Aug;103(4):442-4. doi: 10.3109/13813459509047136.
Phospholipid fatty acid composition and bilirubin UDP-glucuronyltransferase activity from liver microsomal membrane were studied in normal and in bile duct ligated rats. Incubation of normal microsomes with 15 microM bilirubin (considered as physiological concentration) yielded 60% bilirubin diglucuronide; in 2 days post-cholestatic rats, they showed 20% bilirubin diglucuronide which was undetectable in 8 days post-cholestatic group. When compared to controls, after 2 days of cholestasis, microsomal phospholipids showed a clear decrease in linoleic and arachidonic acids and an increment in palmitic and stearic acids. 8 days post-cholestatic rats presented a marked increase in palmitic, oleic and docosaexaenoic acids, while linoleic and arachidonic acids decreased. Cholestasis produced disturbances in microsomal phospholipids fatty acid composition; but these changes are unable to explain entirely the severe damage observed in bilirubin diglucuronide formation.
对正常大鼠和胆管结扎大鼠肝脏微粒体膜的磷脂脂肪酸组成及胆红素UDP-葡萄糖醛酸基转移酶活性进行了研究。用15微摩尔胆红素（视为生理浓度）孵育正常微粒体，生成60%的胆红素二葡萄糖醛酸酯；在胆汁淤积后2天的大鼠中，其胆红素二葡萄糖醛酸酯含量为20%，而在胆汁淤积后8天的大鼠组中未检测到该物质。与对照组相比，胆汁淤积2天后，微粒体磷脂中的亚油酸和花生四烯酸明显减少，棕榈酸和硬脂酸增加。胆汁淤积后8天的大鼠，棕榈酸、油酸和二十二碳六烯酸显著增加，而亚油酸和花生四烯酸减少。胆汁淤积导致微粒体磷脂脂肪酸组成紊乱；但这些变化无法完全解释胆红素二葡萄糖醛酸酯形成过程中所观察到的严重损伤。
