Espíndola-Castro Luís Felipe, de Oliveira Ribeiro Rafael Antonio, de Souza Costa Carlos Alberto, Rosenblatt Aronita, Galembeck André, de Melo Monteiro Gabriela Queiroz
Department of Dentistry, Federal University of Pernambuco (UFPE), Recife, Pernambuco, Brazil.
Department of Dentistry, (FOAr/UNESP), São Paulo State University, Araraquara, São Paulo, Brazil.
Odontology. 2025 Apr;113(2):655-665. doi: 10.1007/s10266-024-01001-4. Epub 2024 Sep 15.
The objective of the study was to assess the indirect cytotoxicity of 600 ppm and 1500 ppm nano silver fluoride (NSF) compared to other commercial cariostatic agents. 56 dentin discs with 0.4 mm in thickness were obtained from intact human molars and adapted to artificial pulp chambers (APCs). The discs were divided into seven groups according to treatment (n = 8): no treatment (positive control-PC), 29% hydrogen peroxide (negative control-NC), 30% Cariestop (CS30), 38% Riva Star (RS38), 38% Advantage Arrest (AA38), 600 ppm NSF (NSF600), and 1500 ppm NSF (NSF1500). The cariostatic agents were applied on the occlusal surface of the dentin discs (facing upward), and the pulp surface (facing downward) remained in contact with the culture medium. Immediately after the treatments, the extracts (DMEM + cariostatic agent components diffused through the discs) were collected and applied to MDPC-23 cells, which were assessed for viability (CV-alamarBlue, live/dead), adhesion/spreading (F-actin), alkaline phosphatase (ALP) activity, and mineralization nodule (MN) formation. The data were statistically analyzed by ANOVA/Games-Howell (p = 0.05). CV and ALP activity in CS30, RS38, AA38, and NSF600 were similar to PC (p > 0.05). MN formation significantly decreased only in NC, CS30, RS38, and AA38 compared to PC (p < 0.001). Only NSF600 and NSF1500 did not differ from PC (p > 0.05) with mineralization nodules, and this specific cell activity significantly decreased in all other groups (p < 0.05). NSF solutions (600 ppm and 1500 ppm) did not cause transdentinal toxicity on MDPC-23 cells.
本研究的目的是评估600 ppm和1500 ppm纳米银氟化物（NSF）与其他商业防龋剂相比的间接细胞毒性。从完整的人类磨牙中获取56个厚度为0.4毫米的牙本质圆盘，并将其适配到人工牙髓腔（APC）中。根据处理方式将圆盘分为七组（n = 8）：未处理（阳性对照-PC）、29%过氧化氢（阴性对照-NC）、30% Cariestop（CS30）、38% Riva Star（RS38）、38% Advantage Arrest（AA38）、600 ppm NSF（NSF600）和1500 ppm NSF（NSF1500）。将防龋剂应用于牙本质圆盘的咬合面（向上），牙髓面（向下）保持与培养基接触。处理后立即收集提取物（DMEM + 通过圆盘扩散的防龋剂成分），并将其应用于MDPC-23细胞，评估细胞活力（CV-alamarBlue，活/死）、黏附/铺展（F-肌动蛋白）、碱性磷酸酶（ALP）活性和矿化结节（MN）形成。数据采用方差分析/盖姆斯-豪厄尔检验进行统计学分析（p = 0.05）。CS30、RS38、AA38和NSF600中的CV和ALP活性与PC相似（p > 0.05）。与PC相比，仅NC、CS30、RS38和AA38中的MN形成显著减少（p < 0.001）。只有NSF600和NSF1500在矿化结节方面与PC无差异（p > 0.05），而在所有其他组中这种特定的细胞活性显著降低（p < 0.05）。NSF溶液（600 ppm和1500 ppm）对MDPC-23细胞未引起穿通牙本质毒性。
