Barnabé Agnès, Loup Benoit, Cawley Adam, Delcourt Vivian, Garcia Patrice, Popot Marie-Agnès, Keledjian John, Bailly-Chouriberry Ludovic
GIE LCH, Laboratoire des Courses Hippiques, Verrières-le-Buisson, France.
Australian Racing Forensic Laboratory, Racing NSW, Sydney, New South Wales, Australia.
Drug Test Anal. 2025 Jul;17(7):989-996. doi: 10.1002/dta.3795. Epub 2024 Sep 16.
Despite the International Federation of Horseracing Authorities (IFHA) regulation associated with heavy sanctions, the abuse of prohibited substances must be identified and deterred throughout horses' athletic careers, such as the administration of recombinant growth hormone (rGH). GH is naturally produced in mammal organisms to stimulate growth. Thus, rGH administration can enhance the performance of horses by expanding some physical abilities. As measuring endogenous GH levels is complex, an indirect strategy is to monitor GH-associated biomarkers in plasma as insulin-like growth factor 1 (IGF-1) levels. To prevent these misuses, the Equine Biological Passport (EBP) has been designed in France (GIE LCH) and Australia (ARFL-Racing NSW) to profile specific biological and chemical parameters in selected racehorses. In this study, we investigated individual limits as a complementary tool to a single limit to supervise the stability of IGF-1 profile over a racing season. The aim is to design custom limits based on the horse's history to detect any deviation below the single limit. The method was assessed using experimental data and then tested on EBP data from three thoroughbreds and three French trotters. Finally, individual limits have been added to the French EBP for IGF-1 monitoring.
尽管国际赛马管理机构联合会（IFHA）制定了相关严厉制裁规定，但在马匹的整个运动生涯中，必须识别并阻止违禁物质的滥用，比如重组生长激素（rGH）的使用。生长激素（GH）是哺乳动物体内自然产生的用于刺激生长的物质。因此，使用rGH可以通过增强某些身体能力来提高马匹的表现。由于测量内源性GH水平较为复杂，一种间接策略是监测血浆中与GH相关的生物标志物，如胰岛素样生长因子1（IGF-1）水平。为防止这些滥用行为，法国（GIE LCH）和澳大利亚（新南威尔士州赛马会-ARFL）设计了马生物护照（EBP），以记录选定赛马的特定生物和化学参数。在本研究中，我们调查了个体限值，作为单一限值的补充工具，以监督IGF-1谱在一个赛季中的稳定性。目的是根据马匹的历史记录设计定制限值，以检测任何低于单一限值的偏差。该方法首先使用实验数据进行评估，然后在来自三匹纯种马和三匹法国快步马的EBP数据上进行测试。最后，个体限值已被添加到法国EBP中用于IGF-1监测。
