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[前列腺素合成酶固定化制剂的分离与特性]

[Isolation and characteristics of immobilized preparations of prostaglandin synthetase].

作者信息

Mevkh A T, Sud'ina G F, Iakusheva L A, Miagkova G I, Evstigneeva R P

出版信息

Biokhimiia. 1985 Jun;50(6):974-9.

PMID:3927991
Abstract

Enzymatic preparations of prostaglandin synthetase were immobilized by various methods, i.e., covalent binding to the carrier, incorporation into the matrix of polyacrylamide gel and adsorption on ion-exchangers. The most representative results were obtained with the use of the absorption technique. In this case, the enzyme activity either decreased very insignificantly or remained unaffected. The stability of immobilized enzyme preparations was studied. Some kinetic characteristics of immobilized prostaglandin synthetase were obtained.

摘要

前列腺素合成酶的酶制剂通过多种方法固定,即与载体共价结合、掺入聚丙烯酰胺凝胶基质以及吸附在离子交换剂上。使用吸附技术获得了最具代表性的结果。在这种情况下,酶活性要么非常轻微地下降,要么保持不变。研究了固定化酶制剂的稳定性。获得了固定化前列腺素合成酶的一些动力学特性。

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Ascorbic acid and prostaglandin formation.
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