Tabatabaei Seyed Mahdi, Varidi Mehdi, Moeenfard Marzieh
Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Food Chem X. 2024 Aug 26;23:101781. doi: 10.1016/j.fochx.2024.101781. eCollection 2024 Oct 30.
Despite poor stability of natural pigments against degradation, using these colorants have attracted great interest due to their various beneficial effect on human health. Accordingly, in the present study, an emulgel based on soy protein isolate-gellan gum conjugate was fabricated via Millard reaction. Then, the effectiveness of emulgel on improving the stability of anthocyanin (ACN) and β-carotene (BC) with different loading concentration (5, 10, and 15 mg/mL) against UV-C irradiation was investigated. Degradation kinetic results exhibited the higher stability of ACN upon co-loading with BC, as the half-life of ACN in free aqueous solution, loaded and co-loaded in emulgel was found to be 0.698, 2.648 and 3.164 days, respectively. The emulgel effectively improved the stability of BC, as well, and no degradation was observed during storage time. The release studies of the pigments showed Fickian diffusion mechanism. Furthermore, their release patterns were found to be independent and differences among the release from individual or simultaneous loaded system were rather small. Overall, our findings elucidated the promising potential of co-loading within emulgel as a safe delivery system in stability enhancement of natural pigments.
尽管天然色素稳定性差，易降解，但因其对人体健康具有多种有益作用，使用这些色素仍引起了广泛关注。因此，在本研究中，通过美拉德反应制备了基于大豆分离蛋白-结冷胶共轭物的乳化凝胶。然后，研究了不同负载浓度（5、10和15mg/mL）的乳化凝胶对提高花青素（ACN）和β-胡萝卜素（BC）抗UV-C辐照稳定性的有效性。降解动力学结果表明，ACN与BC共负载时具有更高的稳定性，因为在游离水溶液中、负载于乳化凝胶中以及与BC共负载于乳化凝胶中的ACN半衰期分别为0.698天、2.648天和3.164天。乳化凝胶也有效提高了BC的稳定性，在储存期间未观察到降解。色素的释放研究表明其符合菲克扩散机制。此外，发现它们的释放模式是独立的，单独负载或同时负载系统的释放差异相当小。总体而言，我们的研究结果阐明了在乳化凝胶中共负载作为一种安全递送系统在提高天然色素稳定性方面的潜在前景。
