Limberis Jason D, Metcalfe John Z
Division of Pulmonary and Critical Care Medicine, Zuckerberg San Francisco General Hospital and Trauma Centre, University of California, San Francisco, San Francisco, CA, USA.
HardwareX. 2024 Aug 27;19:e00576. doi: 10.1016/j.ohx.2024.e00576. eCollection 2024 Sep.
turboLysis is a novel mechanical cell lysis device that utilizes small beads to efficiently lyse tough cells like Mycobacterium, Saccharomyces, and Arabidopsis. We compared turboLysis to bead beating using the BeadBug 6 for several concentrations of roughly correlated to the bacterial load commonly seen in patient samples. turboLysis performed similarly to the BeadBug at low bacterial concentrations and outperformed it at high concentrations above 2x10 CFU/ml (p < 0.005). Thus, turboLysis offers good cell lytic performance in a small form factor at a low cost.
TurboLysis是一种新型的机械细胞裂解装置，它利用小珠子有效地裂解诸如分枝杆菌、酿酒酵母和拟南芥等坚韧的细胞。我们使用BeadBug 6针对几种浓度（大致与患者样本中常见的细菌载量相关）将TurboLysis与珠磨法进行了比较。在低细菌浓度下，TurboLysis的表现与BeadBug相似，而在高于2×10 CFU/ml的高浓度下，其表现优于BeadBug（p < 0.005）。因此，TurboLysis以低成本提供了小尺寸的良好细胞裂解性能。
