Comprehensive analysis of subunit expression, prognostic value, and immune infiltration in clear cell renal cell carcinoma.

BACKGROUND

In recent decades, there has been increasing evidence that Go-Ichi-Nii-San () subunits play an important role in the development and progression of various tumors. However, little research has been conducted on the role of subunits in clear cell renal cell carcinoma (ccRCC). This study sought to explore the differential expression, prognosis, and immunological significance of subunits in ccRCC.

METHODS

We used various analysis packages of R (version 3.6.3), the University of ALabama at Birmingham CANcer (UALCAN) data analysis portal, the Cancer Cell Line Encyclopedia (CCLE), the cBio Cancer Genomics Portal (cBioPortal), and the Tumor Immune Estimation Resource (TIMER) to study the gene expression, promoter methylation level, gene mutations, prognostic and diagnostic value, immune infiltration, pathway enrichment, and other aspects of the subunits. Next, the genes related to the subunits were analyzed using the STRING and GeneMANIA platforms, and the correlation between GINS subunits and the functions involved were investigated.

RESULTS

The expression level of was significantly higher in ccRCC tumor tissues than normal tissues, and was significantly related to tumor grade and stage. The expression of may be related to the methylation degree of the promoter region. The prognostic and diagnostic analyses showed that the increased expression of was associated with various poor prognoses and had diagnostic value. The subunit mutation also significantly affected the clinical prognosis of ccRCC patients. Finally, the correlation analysis of the immune infiltration level, co-expression, and enrichment of related genes indicated that subunit expression was associated with different levels of ccRCC immune infiltration.

CONCLUSIONS

The analysis results showed that the differential expression of subunits in ccRCC, which had prognostic and diagnostic value, was correlated with clinicopathological stage, immune infiltration, and other related aspects. may serve as a new potential prognostic biomarker for ccRCC patients and be used to guide treatment.