Department of General Surgery, Beijing Rehabilitation Hospital Affiliated to Capital Medical University, Xixia Zhuang, Badachu, Shijingshan District, Beijing, China.
Medicine (Baltimore). 2023 Oct 27;102(43):e35681. doi: 10.1097/MD.0000000000035681.
Gastric carcinoma is a common malignant tumor originating from gastric mucosal epithelium. However, role of DS-cell cycle-dependent protein 1 (DSCC1) and GINS1 in gastric carcinoma remains unclear. The gastric carcinoma datasets GSE79973 and GSE118916 were downloaded from gene expression omnibus. Multiple datasets were merged and batched. Differentially expressed genes (DEGs) were screened and weighted gene co-expression network analysis was performed. Functional enrichment analysis, gene set enrichment analysis and immune infiltration analysis were performed. Construction and analysis of protein-protein interaction Network. Survival analysis and comparative toxicogenomics database were performed. A heat map of gene expression was drawn. Target Scan screen miRNAs regulating DEGs. Two thousand forty-four DEGs were identified. According to gene ontology analysis, in biological process, they were mainly enriched in cell migration, transforming growth factor β receptor signaling pathway, angiogenesis, and steroid metabolism process. In cellular component, they were mainly enriched in extracellular vesicles, basement membrane, endoplasmic reticulum lumen, and extracellular space. In molecular function, they focused on extracellular matrix structural components, protein binding, platelet-derived growth factor binding, and catalytic activity. In Kyoto encyclopedia of genes and genomes, they were mainly enriched in protein digestion and absorption, metabolic pathways, fatty acid degradation, Glycerophospholipid metabolism, ether lipid metabolism. Gene set enrichment analysis showed that DEGs were mainly enriched in transforming growth factor β receptor signaling pathway, steroid metabolism process, basement membrane, endoplasmic reticulum lumen, structural components of extracellular matrix, platelet-derived growth factor binding, Glycerophospholipid metabolism, ether lipid metabolism. The results of immune infiltration analysis showed that expression of T cell CD4 memory resting was lower in the samples of gastric cancer. The core genes (TRIP13, CHEK1, DSCC1, GINS1) are protective factors, their expression shows a downward trend with increase of risk score. Comparative toxicogenomics database analysis showed that TRIP13, CHEK1, DSCC1, GINS1 were related to gastric tumors, gastric diseases, tumors, inflammation, and necrosis. DSCC1 and GINS1 are highly expressed in gastric cancer. Higher expression levels of DSCC1 and GINS1, worse the prognosis.
胃癌是一种常见的源于胃黏膜上皮的恶性肿瘤。然而，DS 细胞周期依赖性蛋白 1（DSCC1）和 GINS1 在胃癌中的作用尚不清楚。从基因表达综合数据库中下载了胃癌数据集 GSE79973 和 GSE118916。对多个数据集进行了合并和分批处理。筛选差异表达基因（DEGs），进行加权基因共表达网络分析。进行功能富集分析、基因集富集分析和免疫浸润分析。构建和分析蛋白质-蛋白质相互作用网络。进行生存分析和比较毒理学基因组数据库分析。绘制基因表达热图。靶标扫描筛选调节 DEGs 的 miRNA。鉴定出 2044 个 DEGs。根据基因本体论分析，在生物学过程中，它们主要富集在细胞迁移、转化生长因子β受体信号通路、血管生成和类固醇代谢过程中。在细胞成分中，它们主要富集在细胞外囊泡、基底膜、内质网腔和细胞外空间。在分子功能上，它们集中在细胞外基质结构成分、蛋白质结合、血小板衍生生长因子结合和催化活性上。在京都基因与基因组百科全书中，它们主要富集在蛋白质消化吸收、代谢途径、脂肪酸降解、甘油磷脂代谢、醚脂代谢。基因集富集分析表明，DEGs 主要富集在转化生长因子β受体信号通路、类固醇代谢过程、基底膜、内质网腔、细胞外基质结构成分、血小板衍生生长因子结合、甘油磷脂代谢、醚脂代谢。免疫浸润分析结果表明，胃癌样本中 T 细胞 CD4 记忆静止的表达较低。核心基因（TRIP13、CHEK1、DSCC1、GINS1）是保护因素，其表达随风险评分的增加呈下降趋势。比较毒理学基因组数据库分析表明，TRIP13、CHEK1、DSCC1、GINS1 与胃肿瘤、胃疾病、肿瘤、炎症和坏死有关。DSCC1 和 GINS1 在胃癌中高表达。DSCC1 和 GINS1 表达水平越高，预后越差。
