Kuevi Deryl Nii Okantey, Keiser Jennifer, Häberli Cécile, Owusu-Senyah Abena Konadu, Ahiabu Mawutor Kwame
Council for Scientific and Industrial Research (CSIR), Water Research Institute, Biomedical and Public Health Research Unit, P.O. Box AH 38, Accra, Ghana.
Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.
J Trop Med. 2024 Mar 21;2024:8074291. doi: 10.1155/2024/8074291. eCollection 2024.
Schistosomiasis is caused by parasitic flatworms and the disease is endemic to most countries in sub-Saharan Africa including Ghana. The current therapeutic agent for managing this disease solely relies on praziquantel. The continual dependence on this single available drug could lead to possible drug resistance. This study seeks to evaluate the antischistosomal activity of the following Ghanaian medicinal plants: , , , and . Two concentrations (100 g/mL and 50 g/mL) of each extract were tested in a 96-well plate containing 30 newly transformed schistosomula (NTS). Moreover, six worms of both sexes of adult were exposed to the extracts diluted in the RPMI medium. The assay was performed in a 24-well plate. The parasitic worms were examined using an inverted optical microscope.
At 100 g/mL and 50 g/mL, all extracts performed better and showed strong activity ( < 0.001) against NTS; thus, 98.08%, 100%, 80.77%, and 100% for Clausena, Vernonia, Bridelia, and Khaya, respectively, when compared to praziquantel. Strong activity was recorded when the extracts underwent testing against adults at 100 g/mL; 96.35%, 100%, and 94.55% for Vernonia, Bridelia, and Khaya, respectively, except for Clausena which exhibited weak activity, i.e., 56.02%. There was no significant difference between Vernonia, Bridelia, and Khaya when compared to praziquantel.
At 100 g/mL, , , and extracts demonstrated strong activity against both schistosomula and adult . These data can serve as baseline information in the quest to find alternative therapeutic agents to treat schistosomiasis.
血吸虫病由寄生扁虫引起，该病在包括加纳在内的撒哈拉以南非洲大多数国家流行。目前用于治疗该病的药物仅依赖吡喹酮。持续依赖这单一可用药物可能导致耐药性。本研究旨在评估以下加纳药用植物的抗血吸虫活性： 、 、 和 。在含有30个新转化血吸虫幼虫（NTS）的96孔板中测试了每种提取物的两种浓度（100 g/mL和50 g/mL）。此外，将成年 的六条雌雄虫暴露于在RPMI培养基中稀释的提取物中。该试验在24孔板中进行。使用倒置光学显微镜检查寄生虫。
在100 g/mL和50 g/mL时，所有提取物表现更佳，对NTS显示出强活性（ < 0.001）；因此，与吡喹酮相比， 、 、 和 分别为98.08%、100%、80.77%和100%。当提取物在100 g/mL下对成年 进行测试时记录到强活性； 、 和 分别为96.35%、100%和94.55%，除 表现出弱活性即56.02%外。与吡喹酮相比， 、 和 之间无显著差异。
在100 g/mL时， 、 和 提取物对血吸虫幼虫和成虫均表现出强活性。这些数据可作为寻找治疗血吸虫病替代治疗剂的基线信息。
