Gong Qianyuan, Sun Yueshan, Liu Lei, Pu Chunlan, Guo Yuanbiao
Medical Research Center, The Third People's Hospital of Chengdu, The Affiliated Hospital of Southwest Jiaotong University, Chengdu, 610031, Sichuan, China.
Heliyon. 2024 Aug 23;10(17):e36812. doi: 10.1016/j.heliyon.2024.e36812. eCollection 2024 Sep 15.
Psychological stress disrupts the integrity of the intestinal barrier and is strongly linked to emotional disorders, behavioral changes, and gastrointestinal dysfunction. However, there are limited treatment options available for repairing the damage to the intestinal barrier. As a natural plant-based health beverage, tea () has been shown to have various potentially advantageous effects on the intestinal barrier and immune function. In this study, we extracted bioactive molecules from tea leaves, named exosome-like nanoparticles (ELNs), and then examined their potential protective properties for the intestinal barrier. Through in vitro experimentation, we investigated whether tea-derived ELNs (TELNs) could offer a protective effect against lipopolysaccharides-induced damage to the intestinal barrier. In an in vivo experiment, rats were exposed to water avoidance stress and subsequently administered TELNs orally. The administration of TELNs resulted in the enhancement of the epithelial barrier in the intestine, effectively preventing bacterial translocation to the submucosae. Additionally, TELNs were found to improve the function of the intestinal immune barrier through the mediation of interleukin-22 and the increased secretion of antimicrobial peptide Reg3g. Notably, miR-44 and miR-54 in TELNs exhibited similar protective effects on the intestinal barrier. These findings suggest that TELNs possess the ability to restore the integrity of the intestinal barrier in the context of psychological stress.
心理应激会破坏肠道屏障的完整性，并与情绪障碍、行为改变和胃肠功能障碍密切相关。然而，修复肠道屏障损伤的治疗选择有限。作为一种天然的植物性健康饮品，茶已被证明对肠道屏障和免疫功能具有多种潜在的有益作用。在本研究中，我们从茶叶中提取了生物活性分子，命名为外泌体样纳米颗粒（ELNs），然后研究了它们对肠道屏障的潜在保护特性。通过体外实验，我们研究了茶源ELNs（TELNs）是否能对脂多糖诱导的肠道屏障损伤提供保护作用。在体内实验中，将大鼠暴露于避水应激下，随后口服给予TELNs。给予TELNs可增强肠道上皮屏障，有效防止细菌向黏膜下层移位。此外，发现TELNs通过介导白细胞介素-22和增加抗菌肽Reg3g的分泌来改善肠道免疫屏障的功能。值得注意的是，TELNs中的miR-44和miR-54对肠道屏障表现出类似的保护作用。这些发现表明，TELNs具有在心理应激情况下恢复肠道屏障完整性的能力。
