Steplewski Z, Vogel W H, Ehya H, Poropatich C, Smith J M
Cancer Res. 1985 Oct;45(10):5128-33.
Rats were given injections s.c. of mammary adenocarcinoma cells which developed into undifferentiated carcinomas within a few days. The animals were either left alone or were stressed by restraint for 3 h a day for 11 days and then left for 12 days undisturbed to recover. During this schedule, some animals were sacrificed immediately after the 11-day stress period, whereas others were allowed the 12-day recovery period; unstressed animals were sacrificed as controls on these 2 days. Tumor burden was significantly increased during stress and markedly decreased after the recovery period as compared to unstressed rats. Higher mitotic activity was seen in the tumors of rats which recovered from stress. The immune system responded differently to stress in healthy and tumor-bearing animals. In the tumor-bearing animals, leukocytes were decreased by stress and increased after the recovery period. Lymphocytes were increased, and neutrophiles and large granular lymphocytes were decreased after the recovery period. Total T-cells and suppressor T-cells were decreased during stress and increased during recovery. The percentage of T-cell populations was unaffected by stress, but the percentage of suppressor T-cells increased during recovery. Natural killer cell activity was unaffected by stress but increased after the recovery period. These results indicate that (a) stress and recovery from stress differentially affect tumor development and growth, (b) stress and recovery from stress cause different effects on the immune system in healthy or tumor-bearing animals, (c) stress and recovery from stress stimulate or inhibit different parts of the immune system, and (d) a decreased lymphocyte count and total and suppressor T-cell numbers correlated best with enhanced tumor growth, whereas increased numbers of neutrophils, large granular lymphocytes, total and suppressor T-cells, natural killer cell activity, and a decreased percentage of T-suppressor cells correlated best with depressed tumor growth.
给大鼠皮下注射乳腺腺癌细胞，这些细胞在几天内发展成未分化癌。将动物分为两组，一组不予处理，另一组每天束缚应激3小时，持续11天，然后让其不受干扰地恢复12天。在此期间，一些动物在11天应激期结束后立即处死，而另一些动物给予12天的恢复期；未应激的动物在这两天作为对照处死。与未应激的大鼠相比，应激期间肿瘤负荷显著增加，恢复期后明显降低。从应激中恢复的大鼠肿瘤中可见较高的有丝分裂活性。免疫系统对健康动物和荷瘤动物的应激反应不同。在荷瘤动物中，应激使白细胞减少，恢复期后增加。恢复期后淋巴细胞增加，中性粒细胞和大颗粒淋巴细胞减少。应激期间总T细胞和抑制性T细胞减少，恢复期增加。T细胞群体的百分比不受应激影响，但恢复期抑制性T细胞的百分比增加。自然杀伤细胞活性不受应激影响，但恢复期后增加。这些结果表明：（a）应激和应激恢复对肿瘤发展和生长有不同影响；（b）应激和应激恢复对健康或荷瘤动物的免疫系统有不同影响；（c）应激和应激恢复刺激或抑制免疫系统的不同部分；（d）淋巴细胞计数、总T细胞和抑制性T细胞数量减少与肿瘤生长增强最相关，而中性粒细胞、大颗粒淋巴细胞、总T细胞和抑制性T细胞数量增加、自然杀伤细胞活性增加以及抑制性T细胞百分比降低与肿瘤生长受抑制最相关。
