Sun Jinpeng, Zhang Zhenshan, Xia Binghui, Yao Tianyu, Ge Fengyue, Yan Fengmei
Department of General Surgery Ward,Cangzhou Hospital of Integrated TCM-WM·Hebei China.
Department of Medical Oncology, Cangzhou Hospital of Integrated TCM-WM·Hebei, China.
Heliyon. 2024 Aug 30;10(17):e37062. doi: 10.1016/j.heliyon.2024.e37062. eCollection 2024 Sep 15.
Non-small-cell lung cancer (NSCLC) is the most prevalent form of lung cancer. Circular RNA (circRNA) has emerged as a key player in the development of NSCLC by acting as miRNA sponges. However, the precise role of circ_0114866 in regulating NSCLC process is yet to be elucidated.
The expression of circ_0114866, miR-653-5p, and MYL6B were assessed by qPCR. Cell viability, proliferation, invasion, and migration were investigated using CCK-8, colony formation, Transwell, and wound healing assays. The protein levels of MYL6B, MMP-2, N-cadherin, E-cadherin, and vimentin were evaluated through Western blot analysis. Xenograft tumor model were selected to analyze the impact of circ_0114866 on NSCLC tumor growth. Through circBank or Starbase databases, the binding interactions between miR-653-5p and circ_0114866 or MYL6B were predicted. Subsequently, these interactions were verified by dual-luciferase reporter assay.
The expression of circ_0114866 and MYL6B were clearly elevated, while miR-653-5p expression was notably reduced in NSCLC tissues and cells. Notably, circ_0114866 knockdown obviously suppressed the proliferation, metastasis, and EMT process in NSCLC cells. Additionally, circ_0114866 functioned as a sponge for miR-653-5p, leading to an increase in MYL6B expression by absorbing miR-653-5p. Furthermore, the inhibitory effects on biological behaviors and EMT process of NSCLC cells induced by circ_0114866 knockdown were reversed by miR-653-5p inhibitor. Moreover, experiments demonstrated that silencing circ_0114866 resulted in a repression of tumor growth.
Our findings indicate that circ_0114866 knockdown upregulated MYL6B transcription by sponging miR-653-5p, leading to hinder the progression and EMT process of NSCLC.
非小细胞肺癌（NSCLC）是肺癌最常见的形式。环状RNA（circRNA）通过充当微小RNA（miRNA）海绵，在NSCLC的发展中成为关键因素。然而，circ_0114866在调控NSCLC进程中的具体作用尚待阐明。
通过qPCR评估circ_0114866、miR-653-5p和MYL6B的表达。使用CCK-8、集落形成、Transwell和伤口愈合试验研究细胞活力、增殖、侵袭和迁移。通过蛋白质印迹分析评估MYL6B、基质金属蛋白酶-2（MMP-2）、N-钙黏蛋白、E-钙黏蛋白和波形蛋白的蛋白质水平。选择异种移植肿瘤模型分析circ_0114866对NSCLC肿瘤生长的影响。通过circBank或Starbase数据库预测miR-653-5p与circ_0114866或MYL6B之间的结合相互作用。随后，通过双荧光素酶报告基因试验验证这些相互作用。
NSCLC组织和细胞中circ_0114866和MYL6B的表达明显升高，而miR-６５３-５ｐ表达显著降低。值得注意的是，circ_0114866敲低明显抑制NSCLC细胞的增殖、转移和上皮-间质转化（EMT）过程。此外，circ_0114866充当miR-653-5p的海绵，通过吸收miR-653-5p导致MYL6B表达增加。此外，miR-653-5p抑制剂逆转了circ_0114866敲低对NSCLC细胞生物学行为和EMT过程的抑制作用。此外，实验表明沉默circ_0114866导致肿瘤生长受到抑制。
我们的研究结果表明，circ_0114866敲低通过充当miR-653-5p的海绵上调MYL6B转录，导致阻碍NSCLC的进展和EMT过程。
