Williams J M, Abbud-Filho M, Kelley V E, Strom T B
Cell Immunol. 1985 Sep;94(2):383-93. doi: 10.1016/0008-8749(85)90262-x.
We demonstrate that exposure to highly purified recombinant interleukin 2 (rIL-2) in the absence of known antigenic stimulation induces/increases the expression of two activation-associated proteins, the interleukin 2 receptor (IL-2R) and 4F2 on "resting" peripheral blood mononuclear cells (PBMC); subsequently, these cells enter the S phase of the cell cycle and proliferate. Dual parameter flow cytometry indicates that the phenotype of the cell population(s) which proliferate following this treatment includes HNK+, DR+, and T3+ cells. A twofold expansion in the number of HNK+ and DR+ cells was observed upon culturing resting PBMC in rIL-2 over a 6-day culture period. While the HNK monoclonal antibody is not specific for NK cells, these data do suggest that antigen independent growth of HNK+ cells may represent an additional mechanism by which IL-2 enhances NK effector activity, i.e., by induction of clonal growth of NK cells. In contrast, several concentrations of recombinant Interferon-gamma failed to produce a similar proliferative response.
我们证明，在无已知抗原刺激的情况下，将“静止”外周血单个核细胞（PBMC）暴露于高度纯化的重组白细胞介素2（rIL-2）可诱导/增加两种激活相关蛋白白细胞介素2受体（IL-2R）和4F2的表达；随后，这些细胞进入细胞周期的S期并增殖。双参数流式细胞术表明，经此处理后增殖的细胞群体的表型包括HNK+、DR+和T3+细胞。在rIL-2中培养静止PBMC 6天，观察到HNK+和DR+细胞数量增加了两倍。虽然HNK单克隆抗体对NK细胞不具有特异性，但这些数据确实表明，HNK+细胞的抗原非依赖性生长可能是IL-2增强NK效应活性的另一种机制，即通过诱导NK细胞的克隆生长。相比之下，几种浓度的重组干扰素-γ未能产生类似的增殖反应。
