Prince H E, Kleinman S H, Maino V C, Jackson A L
Cellular Immunology Laboratory, American Red Cross Blood Services, Los Angeles, California 90006.
J Clin Immunol. 1988 Mar;8(2):114-20. doi: 10.1007/BF00917899.
We investigated the relationship of soluble interleukin 2 receptor (sIL2R) production to cellular IL2R expression and DNA synthesis by mitogen-stimulated mononuclear cells from blood donors seropositive for human immunodeficiency virus (HIV). SIL2R was measured using an enzyme-linked immunosorbent assay which employed 2 anti-IL2R monoclonal antibodies recognizing distinct IL2R epitopes. Decreased phytohemagglutinin-induced DNA synthesis and cellular IL2R expression were accompanied by decreased levels of sIL2R in cell culture supernatants. Similar findings were observed for pokeweed mitogen-induced responses. There was no detectable spontaneous secretion of sIL2R into culture supernatants by unstimulated mononuclear cells from either HIV-seropositive or control seronegative donors. These findings indicate that the in vitro T-cell activation defects which characterize HIV infection include decreased sIL2R production, as well as decreased cellular IL2R expression and DNA synthesis. Further, they show that assessment of supernatant sIL2R levels can be used as a valid, reliable assay for T-cell activation.
我们研究了来自人类免疫缺陷病毒（HIV）血清阳性献血者的丝裂原刺激的单核细胞中可溶性白细胞介素2受体（sIL2R）产生与细胞白细胞介素2受体（IL2R）表达及DNA合成之间的关系。使用酶联免疫吸附测定法测量sIL2R，该方法采用两种识别不同IL2R表位的抗IL2R单克隆抗体。植物血凝素诱导的DNA合成及细胞IL2R表达降低，同时细胞培养上清液中sIL2R水平也降低。对于商陆丝裂原诱导的反应也观察到类似的结果。来自HIV血清阳性或对照血清阴性献血者的未刺激单核细胞均未检测到sIL2R自发分泌到培养上清液中。这些发现表明，HIV感染所特有的体外T细胞激活缺陷包括sIL2R产生减少、细胞IL2R表达降低以及DNA合成减少。此外，它们表明上清液sIL2R水平的评估可作为T细胞激活的有效、可靠检测方法。
