Khan Abdur Rafay, Ahmad Bashir, Khan Maleeha, Khan Muhammad Azim, Sultan Shoaib, Sultana Kishwar, Hassan Said
Center of Biotechnology and Microbiology, University of Peshawar, Peshawar 25120, Khyber Pakhtunkhwa, Pakistan.
Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar 25130, Khyber Pakhtunkhwa, Pakistan.
ACS Omega. 2024 Aug 23;9(36):37763-37770. doi: 10.1021/acsomega.4c03317. eCollection 2024 Sep 10.
Food protein deficit has become a major issue worldwide, particularly in underdeveloped countries. Scientists are searching for a variety of less expensive solutions to this issue. One of these less expensive methods is to create single cell protein as a substrate from leftover fruit and vegetable waste, which is typically thrown away. In this regard, the fungal strain (NRRL 21882) was used for the synthesis of SCP (single cell protein) from the waste of banana, potato, and pea. In this manner, 30 samples were collected from the whole substrate with a share of 10 samples each from banana, potato, and pea peels, which were in turn dried and powdered finely. The fermentation process was done by the process of solid state fermentation. (NRRL 21882) generated the highest percentage, i.e. 60.67%, of crude protein from the pea peels. The composition of amino acids in crude proteins was also investigated. The findings demonstrated that the highest percentage of aspartic acid (13.34 ± 0.80%) and glutamic acid (14.92 ± 0.69%) was found in single cell protein produced from pea peels. Soybean was supplemented with single cell protein in the boilers' diet. Compared to all treated groups, there was a substantial ( ≤ 0.05) increase in the level of antibody titer against the Newcastle disease vaccine. The supplementation of single cell protein with soybean meal had no effect on the levels of liver enzymes. The liver enzymes found in all four groups (A, B, C, and D) were within normal limits. None of the examined groups experienced any change in the feed conversion ratio, with all groups exhibiting an average FCR of 1.6. The current study concludes that broiler health and immunity is increased by supplementing poultry feed with single cell protein.
食物蛋白质短缺已成为全球的一个主要问题,尤其是在不发达国家。科学家们正在寻找各种成本较低的解决办法。其中一种成本较低的方法是利用通常被丢弃的水果和蔬菜残余物来生产单细胞蛋白作为底物。在这方面,真菌菌株(NRRL 21882)被用于从香蕉、土豆和豌豆的废弃物中合成单细胞蛋白。通过这种方式,从整个底物中收集了30个样本,其中香蕉、土豆和豌豆皮各10个样本,然后将它们依次干燥并磨成细粉。发酵过程采用固态发酵法。(NRRL 21882)从豌豆皮中产生的粗蛋白百分比最高,即60.67%。还对粗蛋白中的氨基酸组成进行了研究。研究结果表明,在从豌豆皮中产生的单细胞蛋白中,天冬氨酸(13.34±0.80%)和谷氨酸(14.92±0.69%)的百分比最高。在肉鸡日粮中添加单细胞蛋白和豆粕。与所有处理组相比,新城疫疫苗抗体滴度水平有显著(≤0.05)提高。单细胞蛋白与豆粕的添加对肝酶水平没有影响。所有四组(A、B、C和D)中的肝酶均在正常范围内。所有检测组的饲料转化率均无变化,所有组的平均饲料转化率均为1.6。当前研究得出结论,在禽类饲料中添加单细胞蛋白可提高肉鸡的健康水平和免疫力。