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利用废果皮抑制黄曲霉合成黄曲霉毒素:一种用于安全储存大米的生物处理方法。

Utilization of waste fruit-peels to inhibit aflatoxins synthesis by Aspergillus flavus: a biotreatment of rice for safer storage.

机构信息

Department of Chemistry & Biochemistry, University of Agriculture, Faisalabad, Pakistan.

Department of Veterinary Pathology, University of Agriculture, Faisalabad, Pakistan.

出版信息

Bioresour Technol. 2014 Nov;172:423-428. doi: 10.1016/j.biortech.2014.09.017. Epub 2014 Sep 21.

Abstract

Antifungal activity in lemon and pomegranate peels was considerable against Aspergillus flavus, higher in pomegranate (DIZ 37mm; MIC 135μg/mL). Powdered peels (5, 10, 20% w/w) were mixed in inoculated rice. The inhibitory effect on fungal-growth and production of aflatoxins by A. flavus was investigated at storage conditions - temperature (25, 30°C) and moisture (18%, 21%) for 9months. The maximum total aflatoxins accumulated at 30°C, 21% moisture and at 25°C, 18% moisture were 265.09 and 163.45ng/g, respectively in control. Addition of pomegranate-peels inhibited aflatoxins production to 100% during four month-storage of rice at 25°C and 18% moisture, while lemon-peels showed similar inhibitory effect for 3months at same conditions. However a linear correlation was observed in aflatoxins level with temperature and moisture. Studies showed that both fruit-wastes are potent preventer of aflatoxin production in rice, useful for a safer and longer storage of rice.

摘要

柠檬和石榴皮的抗真菌活性对黄曲霉有显著的抑制作用,石榴皮的抑制效果更高(DIZ 37mm;MIC 135μg/mL)。将粉碎的果皮(5%、10%、20%w/w)混入接种的大米中。在 9 个月的贮藏条件下(温度 25、30°C 和水分 18%、21%),研究了其对黄曲霉生长和产生黄曲霉毒素的抑制作用。在 30°C、21%水分和 25°C、18%水分的条件下,对照中总黄曲霉毒素的最大积累量分别为 265.09 和 163.45ng/g。在 25°C 和 18%水分的条件下,石榴皮的添加可在 4 个月的大米贮藏期内抑制黄曲霉毒素的产生,达到 100%,而柠檬皮在相同条件下则可抑制 3 个月。然而,黄曲霉毒素水平与温度和水分呈线性相关。研究表明,这两种水果废料均能有效抑制大米中黄曲霉毒素的产生,有助于大米更安全、更长时间的储存。

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