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针对非生物胁迫的分离物及由……引起的小扁豆根腐病的防治

Isolates Against Abiotic Stresses and Management of Collar rot of Lentil ( L.) Caused by .

作者信息

Meena Nishtha, Yadav D L, Gautam Chirag, Yadav Vinod Kumar, Yadav S L, Meena C B

机构信息

College of Agriculture, Agriculture University, Ummedganj- Kota, Rajasthan India.

Department of Plant Pathology, Agriculture University, Kota, Rajasthan 324001 India.

出版信息

Indian J Microbiol. 2024 Sep;64(3):1366-1375. doi: 10.1007/s12088-024-01356-w. Epub 2024 Jul 27.

DOI:10.1007/s12088-024-01356-w
PMID:39282175
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11399349/
Abstract

A total of 30 native isolates were collected from the Agricultural Research Station, Ummedganj- Kota, Rajasthan, India. Out of which 9 native isolates were evaluated for bio-efficacy against Isolate ARS K-21 exhibited maximum inhibition (89.26%), followed by ARS K-11 (83.70%) in dual culture. Subsequent evaluations revealed the compatibility of efficient isolate ARS K-21 with various bio botanicals displaying minimum inhibition with Vermiwash (1.11-3.70%) followed by Beejamarat (0.38-15.92%) and Brahmastra (7.78-19.68%), while ARS K-11 displayed compatibility only with Dasparni ark with a minimum inhibition of 1.11-3.70%. Assessment of abiotic stress tolerance of the isolates revealed that most isolates thrived at 200 mM and 400 mM NaCl salt concentrations, with ARS K-21 and ARS K-24 demonstrating moderate growth levels across higher concentrations, except at 1200 mM. Optimal growth of the isolates occurred at 25 and 30 °C, with deviations leading to growth inhibition. Isolates ARS K-1, ARS K-11, ARS K-12 and ARS K-21 exhibited resilience to temperature extremes. ARS K-21 has shown exceptional growth proficiency across a wide pH spectrum (pH 5 to 8.5) followed by ARS K- 24, highlighting their versatility. Mass multiplication of efficient isolate ARS K-21 enriched with vermicompost led to the standardization of a dosage (30 g/kg soil) for managing collar rot in lentil crops at 5 g inoculum per kg soil of .

摘要

总共从印度拉贾斯坦邦乌姆梅德甘杰 - 科塔的农业研究站收集了30株本地分离株。其中9株本地分离株针对[具体对象未明确]进行了生物活性评估。在双重培养中,分离株ARS K - 21表现出最大抑制率(89.26%),其次是ARS K - 11(83.70%)。后续评估揭示了高效分离株ARS K - 21与各种生物植物制剂的兼容性,与蚯蚓浸出液表现出最小抑制率(1.11 - 3.70%),其次是碧玛拉特(0.38 - 15.92%)和梵天astra(7.78 - 19.68%),而ARS K - 11仅与达斯帕尼方舟表现出兼容性,最小抑制率为1.11 - 3.70%。对分离株的非生物胁迫耐受性评估表明,大多数分离株在200 mM和400 mM NaCl盐浓度下生长良好,ARS K - 21和ARS K - 24在较高浓度下表现出中等生长水平,但在1200 mM时除外。分离株的最佳生长温度为25和30°C,温度偏差会导致生长抑制。分离株ARS K - 1、ARS K - 11、ARS K - 12和ARS K - 21对极端温度具有耐受性。ARS K - 21在较宽的pH谱(pH 5至8.5)中表现出卓越的生长能力,其次是ARS K - 24,突出了它们的多功能性。用蚯蚓堆肥富集的高效分离株ARS K - 21进行大规模繁殖,得出了在扁豆作物中防治根腐病的剂量标准(30 g/kg土壤),每千克土壤接种量为5 g。