Zhang Chenchen, Wu Zhuhua, Huang Xinchun, Zhao Yuchuan, Sun Qi, Chen Yanmei, Guo Huixin, Liao Qinghua, Wu Huizhong, Chen Xunxun, Liang Anqi, Dong Wenya, Yu Meiling, Chen Yuhui, Wei Wenjing
Center for Tuberculosis Control of Guangdong Province, Guangzhou, 510630 China.
Present Address: Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China.
Indian J Microbiol. 2024 Sep;64(3):1044-1056. doi: 10.1007/s12088-024-01236-3. Epub 2024 Mar 20.
Guangdong Province, China's largest economy, has a high incidence of tuberculosis (TB). At present, there are few reports on the distribution, transmission and drug resistance of (Mtb) strains in this region. In this study, we performed minimum inhibitory concentration testing for 14 anti-TB drugs and whole-genome sequencing of 713 clinical Mtb isolates from 20,662 sputum culture-positive tuberculosis patients registered at 31 tuberculosis drug resistance surveillance sites covering 20 cities in Guangdong Province from 2016 to 2018. Moreover, we evaluated genome-wide associations between mutations and drug resistance, and further investigated the differences in the MICs of mutations. The epidemiology, drug-resistant phenotypes and whole genome sequencing data of 713 clinical Mtb isolates were analyzed, revealing the lineage distribution and drug-resistant gene profiles in Guangdong Province. WGS combined with quantitative MIC measurements identified several novel loci associated with resistance, of which 16 loci were found to be related to resistance to more than one drug. This study analyzed the lineage distribution, prevalence characteristics and resistance-corresponding gene profiles of Mtb isolates in Guangdong province, and provided a theoretical basis for the formulation of tuberculosis prevention and control policy in the province.
The online version contains supplementary material available at 10.1007/s12088-024-01236-3.
中国经济最发达的广东省结核病发病率较高。目前，关于该地区结核分枝杆菌（Mtb）菌株的分布、传播和耐药性的报道较少。在本研究中，我们对14种抗结核药物进行了最低抑菌浓度测试，并对2016年至2018年期间在广东省20个城市的31个结核病耐药监测点登记的20662例痰培养阳性结核病患者的713株临床Mtb分离株进行了全基因组测序。此外，我们评估了突变与耐药性之间的全基因组关联，并进一步研究了突变的最低抑菌浓度差异。分析了713株临床Mtb分离株的流行病学、耐药表型和全基因组测序数据，揭示了广东省的谱系分布和耐药基因谱。全基因组测序结合定量最低抑菌浓度测量确定了几个与耐药性相关的新位点，其中16个位点与对多种药物的耐药性有关。本研究分析了广东省Mtb分离株的谱系分布、流行特征和耐药相关基因谱，为该省结核病防控政策的制定提供了理论依据。
在线版本包含可在10.1007/s12088-024-01236-3获取的补充材料。
