Zhu Jinghui, Lim Kean-Jin, Fang Tianyu, Zhang Chen, Ye Jianren, Zhu Li-Hua
College of Forestry and Grassland, Nanjing Forestry University, Nanjing, Jiangsu 210037, China.
State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A&F University, Lin'an District, Hangzhou, Zhejiang 311300, China.
Phytopathology. 2024 Dec;114(12):2525-2535. doi: 10.1094/PHYTO-06-24-0180-R. Epub 2024 Dec 16.
Pine wilt disease (PWD) is caused by the pine wood nematode (PWN, ) and significantly impacts pine forest ecosystems globally. This study focuses on , an important timber and oleoresin resource in China, which is highly susceptible to PWN. However, the defense mechanism of pine trees in response to PWN remains unclear. Addressing the complexities of PWD, influenced by diverse factors such as bacteria, fungi, and environment, we established a reciprocal system between PWN and seedlings under aseptic conditions. Utilizing combined second- and third-generation sequencing technologies, we identified 3,718 differentially expressed genes post PWN infection. Transcript analysis highlighted the activation of defense mechanisms via stilbenes, salicylic acid and jasmonic acid pathways, terpene synthesis, and induction of pathogenesis-related proteins and resistance genes, predominantly at 72 h postinfection. Notably, terpene synthesis pathways, particularly the mevalonate pathway, were crucial in defense, suggesting their significance in 's response to PWN. This comprehensive transcriptome profiling offers insights into 's intricate defense strategies against PWN under aseptic conditions, laying a foundation for future functional analyses of key resistance genes. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.
松材线虫病（PWD）由松材线虫（PWN）引起，对全球松林生态系统造成重大影响。本研究聚焦于中国一种重要的木材和松脂资源[具体树种未给出]，该树种对PWN高度敏感。然而，松树对PWN的防御机制仍不清楚。针对受细菌、真菌和环境等多种因素影响的松材线虫病的复杂性，我们在无菌条件下建立了PWN与[具体树种]幼苗之间的互作体系。利用二代和三代测序技术相结合，我们鉴定出PWN感染后3718个差异表达基因。转录分析表明，主要在感染后72小时，通过芪类、水杨酸和茉莉酸途径、萜类合成以及病程相关蛋白和抗性基因的诱导激活了防御机制。值得注意的是，萜类合成途径，尤其是甲羟戊酸途径，在防御中至关重要，表明它们在[具体树种]对PWN的反应中具有重要意义。这种全面的转录组分析揭示了无菌条件下[具体树种]对PWN的复杂防御策略，为未来关键抗性基因的功能分析奠定了基础。[公式：见正文] 版权所有© 2024作者。本文是一篇根据知识共享署名4.0国际许可协议分发的开放获取文章。
