Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
Faculty of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2023 Jan 17;24(3):1812. doi: 10.3390/ijms24031812.
Lamb. is a crucial timber and resin conifer in China, but its plantation industry is threatened by outbreaks of pine wilt disease (PWD) caused by (pinewood nematode; PWN). However, as of yet, there is no comprehensive analysis of NBS-LRR genes in involved in its defense against PWN. In this study, 507 NBS genes were identified in the transcriptome of resistant and susceptible inoculated with the PWN. The phylogenetic analysis and expression profiles of resistant and susceptible revealed that the up-regulated gene was involved in conferring resistance to PWN. The results of real-time quantitative PCR (qRT-PCR) showed that was significantly up-regulated after PWN infection, especially in the stems. Subcellular localization indicated that PmNBS-LRR97 located to the cell membrane. significantly activated the expression of reactive oxygen species (ROS)-related genes in . In addition, the overexpression of was capable of promoting the production of ROS, aiding in plant growth and development. In summary, participates in the defense response to PWN and plays an active role in conferring resistance in . This finding provides new insight into the regulatory mechanism of the R gene in .
油松是中国重要的用材和树脂针叶树种，但由于松材线虫（PWN）引起的松树萎蔫病（PWD）的爆发，其种植产业受到威胁。然而，迄今为止，尚未对 中参与防御 PWN 的 NBS-LRR 基因进行全面分析。在这项研究中，从接种 PWN 的抗性和敏感 转录组中鉴定出 507 个 NBS 基因。抗性和敏感 的系统发育分析和表达谱表明，上调的 基因参与赋予对 PWN 的抗性。实时定量 PCR（qRT-PCR）的结果表明，PWN 感染后 明显上调，尤其是在茎中。亚细胞定位表明 PmNBS-LRR97 位于细胞膜上。 可显著激活 中与活性氧（ROS）相关基因的表达。此外， 的过表达能够促进 ROS 的产生，有助于植物的生长和发育。总之， 参与了对 PWN 的防御反应，并在 中发挥了积极的抗性作用。这一发现为 中 R 基因的调控机制提供了新的见解。
