Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Khuzestan Province, Iran.
Biotechnology and Bioscience Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Antonie Van Leeuwenhoek. 2024 Sep 16;118(1):5. doi: 10.1007/s10482-024-02019-y.
Identification of Fusarium species associated with diseases symptoms in plants is an important step toward understanding the ecology of plant-fungus associations. In this study, four Fusarium isolates were obtained from root rot of Oryza sativa L. in Izeh (southwest of Iran) and identified based on phylogenetic analyses combined with morphology. Phylogenetic analyses based on combined translation elongation factor 1-α, calmodulin, RNA polymerase II second largest subunit, and Beta-tubulin (tub2) sequence data delimited two new species, namely F. khuzestanicum and F. oryzicola spp. nov., from previously known species of Fusarium within F. incarnatum-equiseti species complex (FIESC). Morphologically, F. khuzestanicum produces the macroconidia with distinctly notched to foot-shaped basal cells, while basal cells in the macroconidia of F. oryzicola are more extended and distinctly elongated foot shape. Furthermore, these two new species are distinguished by the size of their sporodochial phialides and macroconidia. The results of the present show that the FIESC species complex represent more cryptic species.
鉴定与植物病害症状相关的镰刀菌物种是了解植物-真菌共生关系生态学的重要步骤。在这项研究中，从伊朗西南部伊泽（Izeh）的水稻根腐病中获得了四个镰刀菌分离株，并基于形态学结合系统发育分析进行了鉴定。基于翻译延伸因子 1-α、钙调蛋白、RNA 聚合酶 II 第二大亚基和 Beta-微管蛋白（tub2）序列数据的系统发育分析，将两个新物种，即 F. khuzestanicum 和 F. oryzicola spp. nov.，从已知的镰刀菌属 F. incarnatum-equiseti 种复合体（FIESC）中鉴定出来。形态上，F. khuzestanicum 产生具有明显缺口到足形基细胞的大型分生孢子，而 F. oryzicola 的大型分生孢子的基细胞则更长，明显呈足形伸长。此外，这两个新种还可以通过其孢子梗瓶梗和大型分生孢子的大小来区分。本研究结果表明，FIESC 种复合体代表更多的隐种。
