基于双导电聚合物纳米线的电化学适体传感器用于唾液中皮质醇的高灵敏、低污染检测。
A double-conducting polymer nanowire-based electrochemical aptasensor for highly sensitive and low fouling detection of cortisol in saliva.
机构信息
Qingdao Agricultural University, Qingdao, China.
School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, Yantai, China.
出版信息
Mikrochim Acta. 2024 Sep 16;191(10):600. doi: 10.1007/s00604-024-06695-5.
A cortisol biosensor was developed based on double-conducting polymer nanowires, which exhibits excellent conductivity, resistance to biological contamination, and outstanding sensing performance. The biosensor employs dual-mode electrochemical techniques, namely, differential pulse voltammetry (DPV) and chronoamperometry (CA), for the sensitive and low fouling detection of the glucocorticoid hormone cortisol. Experimental results demonstrated that the linear detection range of the biosensor in DPV mode was 1.0 × 10-1.0 × 10 M, with a detection limit of 0.131 × 10 M. In CA mode, the biosensor exhibited a detection range of 1.0 × 10-1.0 × 10 M and a detection limit of 0.313 × 10 M. The biosensor was successfully utilized for the rapid detection of cortisol in human saliva. The combination of a high-specificity cortisol aptamer and functionalized double-conducting polymer nanowires ensured the exceptional specificity and sensitivity of the biosensor in detecting real biological samples.
一种基于双导体制聚合物纳米线的皮质醇生物传感器被开发出来，它具有出色的导电性、抗生物污染性和卓越的传感性能。该生物传感器采用双模式电化学技术，即差分脉冲伏安法（DPV）和计时安培法（CA），用于灵敏且低污染地检测糖皮质激素皮质醇。实验结果表明，该生物传感器在 DPV 模式下的线性检测范围为 1.0×10-1.0×10 M，检测限为 0.131×10 M。在 CA 模式下，该生物传感器的检测范围为 1.0×10-1.0×10 M，检测限为 0.313×10 M。该生物传感器成功地用于快速检测人唾液中的皮质醇。皮质醇适体与功能化双导体制聚合物纳米线的结合确保了该生物传感器在检测真实生物样本时具有出色的特异性和灵敏度。
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