Graduate School of Health Sciences, Istanbul University, Istanbul, Turkey.
Faculty of Medicine, Department of Histology and Embryology, Giresun University, Giresun, Turkey.
J Mol Histol. 2024 Dec;55(6):1211-1223. doi: 10.1007/s10735-024-10261-x. Epub 2024 Sep 16.
Despite the negative environmental and biologic effects, organophosphates have currently been widely used. We aimed to examine the possible negative effects of diazinon, a type of organophosphate, on rat ovarian tissue. Wistar Albino rats were divided into four groups. No treatment was given to control, olive oil was applied to sham group. Experimental groups were injected intraperitoneally with 30 and 60 mg/kg/day diazinon, respectively. 24 h later, ovarian tissues were extracted, preparated, examined via light and electron microscope. In the experimental groups granulosa and corpus luteum showed degenerative changes. Dilatation of endoplasmic reticulum cisterns and morphological alterations of mitochondria in granulosa cells were detected utrastructurally. Also, accumulation of lipid droplets and autophagic vacuoles was observed in cells of corpus luteum. A statistically significant dose-dependent decrease in superoxide dismutase and catalase reactivity and a statistically significant increase in caspase-3 expression in cells of atretic follicles and corpus luteum were observed. Results show that exposure to a single dose of diazinon may disrupt antioxidant system, trigger atresia in follicles and negatively effect corpus luteum functions. It was concluded that studies applying possible antioxidant treatments should be carried out to reduce and prevent the negative effects of diazinon on the reproductive system.
尽管有机磷化合物具有负面的环境和生物学效应，但目前已被广泛应用。本研究旨在探讨一种有机磷化合物——二嗪磷对大鼠卵巢组织可能产生的负面影响。将 Wistar 白化大鼠分为四组：对照组不做任何处理，假手术组给予橄榄油，实验组分别给予 30 和 60mg/kg/天的二嗪磷腹腔注射。24 小时后提取卵巢组织，通过光镜和电镜进行检查。实验组的颗粒细胞和黄体出现退行性变化。超微结构观察到颗粒细胞内质网池扩张和线粒体形态改变，黄体细胞中出现脂滴和自噬空泡堆积。在闭锁卵泡和黄体细胞中，细胞色素 C 表达水平显著升高，而过氧化物歧化酶和过氧化氢酶的活性显著降低，且呈剂量依赖性。结果表明，单次接触二嗪磷可能会破坏抗氧化系统，导致卵泡闭锁，并对黄体功能产生负面影响。因此，应该开展应用抗氧化治疗的研究，以减少和预防二嗪磷对生殖系统的负面影响。
