Shvartsman P Ia, Bondarenko L V, Romashkina T B
Genetika. 1985 Jun;21(6):958-63.
The rate of recessive sex-linked lethal mutations (RLM) was estimated by brood pattern method at different stages of oogenesis, initially, in the wild-type R-86 strain of Drosophila melanogaster after treatment with EI and EMS. The former which is known to induce dominant lethals in mature oocytes of the 14th stage with a high frequency was equally effective in inducing RLM in oocytes of different age and in oogonia. EMS which does not induce dominant lethals when used as vapour was shown to increase RLM frequency in mature fraction of oocytes (the 14A stage only). Similar type of different mutability was found in mutagen-sensitive strain mus-201G1 and in the control 3-4 strain having the same genetical background as mus mutation. Female germ cells of mus-201G1 strain appeared to have a higher mutability in the case of EI, though no differences in mutability between these strains after EMS treatment were registered. The data are discussed in view of the specificity of primer damages occurring as a result of comparable mutagens action and participation of different repair systems in elimination of these damages.
采用育雏模式法，在不同卵子发生阶段，最初是在用EI和EMS处理后的野生型黑腹果蝇R-86品系中，估算隐性性连锁致死突变（RLM）的发生率。已知前者能在第14阶段的成熟卵母细胞中高频诱导显性致死突变，在不同年龄的卵母细胞和卵原细胞中诱导RLM同样有效。用作蒸汽时不诱导显性致死突变的EMS，被证明能增加成熟卵母细胞部分（仅14A阶段）的RLM频率。在诱变敏感品系mus-201G1和与mus突变具有相同遗传背景的对照3-4品系中，发现了类似的不同诱变率类型。在EI处理的情况下，mus-201G1品系的雌性生殖细胞似乎具有更高的诱变率，不过在EMS处理后，这些品系之间的诱变率没有差异。鉴于可比诱变剂作用导致的引物损伤的特异性以及不同修复系统在消除这些损伤中的参与情况，对这些数据进行了讨论。
