Fliss E R, Setlow P
Gene. 1985;35(1-2):151-7. doi: 10.1016/0378-1119(85)90167-2.
The complete nucleotide (nt) sequence of two Bacillus megaterium genes coding for small, acid-soluble spore proteins (SASP), termed C-1 and C-2, has been determined. The nt sequences of the genes are greater than 98% identical in the coding regions, greater than 90% identical in approx. 180 bp and approx. 50 bp of upstream and downstream flanking sequences, respectively, and exhibit features conserved in related B. megaterium SASP genes. Northern blot analyses showed that the SASP-C-1 and/or C-2 genes are transcribed during sporulation in parallel with the related SASP-C and C-3 genes. The promoter regions of the SASP-C-1 and C-2 genes were localized, based on the sizes of their mRNAs and the positions of transcription termination sequences. The SASP-C-1 and C-2 genes' promoter regions exhibit significant homology with those for the SASP-C and C-3 genes.
已确定编码小酸溶性芽孢蛋白（SASP）的两个巨大芽孢杆菌基因（称为C-1和C-2）的完整核苷酸（nt）序列。这些基因的nt序列在编码区的同一性大于98%，在上游和下游侧翼序列分别约180 bp和约50 bp的区域中同一性大于90%，并且具有在相关巨大芽孢杆菌SASP基因中保守的特征。Northern印迹分析表明，SASP-C-1和/或C-2基因在芽孢形成过程中与相关的SASP-C和C-3基因平行转录。基于其mRNA的大小和转录终止序列的位置，确定了SASP-C-1和C-2基因的启动子区域。SASP-C-1和C-2基因的启动子区域与SASP-C和C-3基因的启动子区域具有显著的同源性。
