Fliss E R, Setlow P
Gene. 1984 Oct;30(1-3):167-72. doi: 10.1016/0378-1119(84)90117-3.
The nucleotide sequence of the Bacillus megaterium gene coding for spore-specific protein C-3 has been determined. The gene codes for 65 amino acids and the coding sequence is preceded by an efficient ribosome-binding site. The predicted protein C-3 sequence agrees with both the amino acid composition and the amino terminal sequence of protein C-3, and shows homology (approx. 65% of all residues are identical) with the sequences of the analogous proteins A and C of B. megaterium. Protein C-3 is cleaved by the sequence-specific B. megaterium spore protease, and the amino acid sequence at the new amino-terminus generated is identical to that predicted from the gene sequence, and homologous to the spore protease cleavage sites in the A and C proteins. The protein C-3 gene also shares a number of features with the previously sequenced protein C gene in both upstream and downstream flanking sequence.
已确定编码巨大芽孢杆菌孢子特异性蛋白C-3的基因的核苷酸序列。该基因编码65个氨基酸，编码序列之前有一个有效的核糖体结合位点。预测的蛋白C-3序列与蛋白C-3的氨基酸组成和氨基末端序列均相符，并且与巨大芽孢杆菌类似蛋白A和C的序列具有同源性（约65%的所有残基相同）。蛋白C-3被序列特异性的巨大芽孢杆菌孢子蛋白酶切割，产生的新氨基末端的氨基酸序列与从基因序列预测的序列相同，并且与A和C蛋白中的孢子蛋白酶切割位点同源。蛋白C-3基因在上下游侧翼序列方面也与先前测序的蛋白C基因有许多共同特征。
