Developing Brain Institute, Center for Prenatal, Neonatal and Maternal Health Research, Children's Research Institute, Children's National Hospital, Washington, DC, USA.
Department of Pediatrics, The George Washington University, Washington, DC, USA.
Clin Epigenetics. 2024 Sep 16;16(1):128. doi: 10.1186/s13148-024-01741-8.
To assess the impact of postnatal processing on placental DNA methylation, array data from flash-frozen placental tissue was compared to perfluorocarbon-immersed and formalin-fixed paraffin-embedded placental tissue. We observed that tissue exposed to perfluorocarbon showed no significant DNA methylation differences when compared to unprocessed tissue, while formalin processing altered the quality and reliability of the data produced on the DNA methylation array platform. Placental DNA methylation allows for the study of gene-environment interactions that influence the fetal environment and development. Our study highlights that placental post-processing techniques must be considered in the evaluation and interpretation of epigenetic studies.
为了评估产后处理对胎盘 DNA 甲基化的影响，我们比较了来自快速冷冻胎盘组织的阵列数据与全氟碳浸泡和福尔马林固定石蜡包埋胎盘组织的阵列数据。我们观察到，与未经处理的组织相比，暴露于全氟碳的组织没有显示出明显的 DNA 甲基化差异，而福尔马林处理改变了 DNA 甲基化阵列平台上产生的数据的质量和可靠性。胎盘 DNA 甲基化允许研究影响胎儿环境和发育的基因-环境相互作用。我们的研究强调，在评估和解释表观遗传学研究时，必须考虑胎盘产后处理技术。
