Use of a phoB'-'lacZ fusion gene to determine the N-terminal amino acid sequence of the phoB protein and to prepare antiserum against the protein.
作者信息
Sugita T, Shinagawa H, Makino K, Nakata A
出版信息
J Biochem. 1985 Apr;97(4):1247-50. doi: 10.1093/oxfordjournals.jbchem.a135170.
Abstract
phoB is a positive regulatory gene of the phosphate regulon of Escherichia coli. A plasmid carrying a phoB'-'lacZ fusion gene was constructed by in vitro recombination. A PhoB-LacZ hybrid protein was purified from the cells carrying the plasmid by monitoring beta-galactosidase activity. The amino-terminal amino acid sequence of the PhoB protein was determined by utilizing the hybrid protein. Antiserum against the PhoB protein was prepared by immunizing rabbits with the hybrid protein. The serum thus prepared showed specificity for both the PhoB protein and beta-galactosidase.
摘要
相似文献
1
Use of a phoB'-'lacZ fusion gene to determine the N-terminal amino acid sequence of the phoB protein and to prepare antiserum against the protein.
J Biochem. 1985 Apr;97(4):1247-50. doi: 10.1093/oxfordjournals.jbchem.a135170.
2
Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2.PhoM蛋白与大肠杆菌磷酸调节子的相互作用：PhoM是一种组氨酸蛋白激酶，催化PhoB和PhoM开放阅读框2的磷酸化。
J Bacteriol. 1990 Nov;172(11):6300-7. doi: 10.1128/jb.172.11.6300-6307.1990.
3
Regulation of the pho regulon in Escherichia coli K-12. Genetic and physiological regulation of the positive regulatory gene phoB.大肠杆菌K-12中pho操纵子的调控。正调控基因phoB的遗传与生理调控。
J Mol Biol. 1983 Aug 15;168(3):477-88. doi: 10.1016/s0022-2836(83)80297-6.
4
Nucleotide sequence of the phoB gene, the positive regulatory gene for the phosphate regulon of Escherichia coli K-12.大肠杆菌K-12磷酸调节子的正调控基因phoB的核苷酸序列。
J Mol Biol. 1986 Jul 5;190(1):37-44. doi: 10.1016/0022-2836(86)90073-2.
5
Regulation of the phosphate regulon of Escherichia coli K-12: regulation and role of the regulatory gene phoR.大肠杆菌K-12磷酸盐调节子的调控：调控基因phoR的调控作用
J Mol Biol. 1985 Jul 20;184(2):231-40. doi: 10.1016/0022-2836(85)90376-6.
6
Cloning and characterization of the alkaline phosphatase positive regulatory gene (phoM) of Escherichia coli.大肠杆菌碱性磷酸酶正调控基因（phoM）的克隆与特性分析
Mol Gen Genet. 1984;195(3):381-90. doi: 10.1007/BF00341438.
7
Regulation of the phosphate regulon of Escherichia coli: analysis of mutant phoB and phoR genes causing different phenotypes.大肠杆菌磷酸调节子的调控：对导致不同表型的突变型phoB和phoR基因的分析。
J Bacteriol. 1989 Oct;171(10):5601-6. doi: 10.1128/jb.171.10.5601-5606.1989.
8
New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase.新型多功能质粒载体，用于表达由与编码β-半乳糖苷酶酶活性羧基末端部分的lacZ基因序列融合的克隆基因所编码的杂交蛋白。
Gene. 1983 Nov;25(1):71-82. doi: 10.1016/0378-1119(83)90169-5.
9
Regulation of the lkyB gene expression in Escherichia coli K-12 strains carrying an lkyB-lacZ gene fusion.
Mol Gen Genet. 1985;201(2):323-8. doi: 10.1007/BF00425678.
10
Multiple controls exerted on in vivo expression of the pepN gene in Escherichia coli: studies with pepN-lacZ operon and protein fusion strains.对大肠杆菌中pepN基因体内表达的多种调控：pepN - lacZ操纵子和蛋白质融合菌株的研究
J Bacteriol. 1985 Sep;163(3):1191-5. doi: 10.1128/jb.163.3.1191-1195.1985.
Zotero 插件