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与昆虫病原线虫相关的共生细菌对吸虫寄生虫中间宿主福寿螺和钉螺的杀螺特性。

Molluscicidal property of symbiotic bacteria associated with entomopathogenic nematodes against and , the intermediate hosts of trematode parasites.

作者信息

Dumidae Abdulhakam, Homkeaw Chanatinart, Subkrasae Chanakan, Ardpairin Jiranun, Pansri Supawan, Polseela Raxsina, Phoungpetchara Ittipon, Kumchantuek Tewarat, Tandhavanan Sarunporn, Thanwisai Aunchalee, Vitta Apichat

机构信息

Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.

Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.

出版信息

Parasite Epidemiol Control. 2024 Aug 28;27:e00375. doi: 10.1016/j.parepi.2024.e00375. eCollection 2024 Nov.

Abstract

and act as intermediate hosts for veterinary and medical trematode parasites. Snail control is a strategy used to decrease the number of snails and interrupt the life cycle of parasites. The objective of this study was to evaluate the efficacy of and extracts against and in the laboratory. Ethyl acetate extracts of selected symbiotic bacteria were tested for their molluscicidal activities according to World Health Organization guidelines. Additionally, pathological changes in the snails were observed after treatment with the LC50 values under a light microscope. and were susceptible to all ethyl acetate extracts of symbiotic bacteria. The lowest LC50 and LC90 at 24 h for after exposure to subsp. (bALN18.2_TH) extracts were 81.66 and 151.02 ppm, respectively. Similarly, the lowest LC50 and LC90 at 24 h for after exposure to subsp. (bAPY3.5_TH) extracts were 49.21 and 147.66 ppm, respectively. species had more substantial molluscicidal effects than on these snails. The ethyl acetate extracts of these bacteria are effective when contacting the epithelial cells and foot muscle of the snails. To our knowledge, this is the first report on using and extracts to evaluate molluscicidal activities. These symbiotic bacteria, and may be useful for controlling snail intermediate hosts.

摘要

并作为兽医和医学吸虫寄生虫的中间宿主。控制蜗牛是一种用于减少蜗牛数量并中断寄生虫生命周期的策略。本研究的目的是在实验室中评估[具体细菌名称1]和[具体细菌名称2]提取物对[蜗牛品种1]和[蜗牛品种2]的功效。根据世界卫生组织的指导方针,对选定共生细菌的乙酸乙酯提取物进行了杀螺活性测试。此外,在用LC50值处理后,在光学显微镜下观察蜗牛的病理变化。[蜗牛品种1]和[蜗牛品种2]对所有共生细菌的乙酸乙酯提取物均敏感。暴露于[具体细菌亚种1](bALN18.2_TH)提取物后,[蜗牛品种1]在24小时时的最低LC50和LC90分别为81.66和151.02 ppm。同样,暴露于[具体细菌亚种2](bAPY3.5_TH)提取物后,[蜗牛品种2]在24小时时的最低LC50和LC90分别为49.21和147.66 ppm。[细菌品种1]对这些蜗牛的杀螺作用比[细菌品种2]更显著。这些细菌的乙酸乙酯提取物在与蜗牛的上皮细胞和足部肌肉接触时是有效的。据我们所知,这是关于使用[具体细菌名称1]和[具体细菌名称2]提取物评估杀螺活性的首次报告。这些共生细菌,[具体细菌名称1]和[具体细菌名称2]可能有助于控制蜗牛中间宿主。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e797/11402155/b6a2e12addd1/gr1.jpg

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