Saijuntha Weerachai, Tantrawatpan Chairat, Agatsuma Takeshi, Rajapakse R P V Jayanthe, Karunathilake K J K, Pilap Warayutt, Tawong Wittaya, Petney Trevor N, Andrews Ross H
Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, Thailand.
Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, and Center of Excellence in Stem Cell Research, Thammasat University, Rangsit Campus, Pathum Thani 12120, Thailand.
One Health. 2021 Jan 6;12:100211. doi: 10.1016/j.onehlt.2021.100211. eCollection 2021 Jun.
The freshwater snail play an important role as the sole intermediate host of several medically- and economically-important trematodes, especially zoonotic schistosomes and echinostomes which can infect and cause diseases in livestock and people. This study aims to explore the mitochondrial cytochrome oxidase subunit 1 sequence variation of collected from new geographical areas; 459 specimens of were collected from 43 localities in South and Southeast Asia. The 42 haplotypes (Ie1 - Ie42) we detected were classified into haplogroups I - V. Phylogenetic analyses revealed five major clades, A - E, in concordance with all previous studies. Clade E contained two subclades, E1 (haplogroup I) and E2 (haplogroup II). The most widespread genetic group was subclade E1. Clade A, clade B (haplogroup V), and clade C (haplogroup IV) were found only in South Asia, whereas clade D (haplogroup III) was specifically found in Southeast Asia. In Thailand, showed high genetic divergence with 21 haplotypes. Several isolates showed significant genetic differences from others with unique haplotype(s). Hence, we confidently conclude our findings support all previous studies that is a species complex with at least four major lineages and five haplogroups. Our additional analyses of 35 samples from Sri Lanka showed these were indeed an independent genetic group as previously found, but they can now be classified as a unique group forming subclade E2 (haplogroup II) of sensu lato.
淡水螺作为几种具有医学和经济重要性的吸虫的唯一中间宿主发挥着重要作用，特别是人畜共患的血吸虫和棘口吸虫，它们可感染家畜和人类并引发疾病。本研究旨在探索从新地理区域收集的[淡水螺名称未给出]的线粒体细胞色素氧化酶亚基1序列变异；从南亚和东南亚的43个地点收集了459个[淡水螺名称未给出]标本。我们检测到的42个单倍型（Ie1 - Ie42）被分为单倍型类群I - V。系统发育分析揭示了五个主要分支，A - E，与之前所有研究一致。分支E包含两个亚分支，E1（单倍型类群I）和E2（单倍型类群II）。分布最广的遗传群体是亚分支E1。分支A、分支B（单倍型类群V）和分支C（单倍型类群IV）仅在南亚发现，而分支D（单倍型类群III）专门在东南亚发现。在泰国，[淡水螺名称未给出]表现出高度的遗传分化，有21个单倍型。几个分离株与其他分离株表现出显著的遗传差异，具有独特的单倍型。因此，我们有信心得出结论，我们的研究结果支持之前所有的研究，即[淡水螺名称未给出]是一个物种复合体，至少有四个主要谱系和五个单倍型类群。我们对来自斯里兰卡的35个样本的额外分析表明，这些样本确实如之前所发现的那样是一个独立的遗传群体，但现在它们可被归类为一个独特的群体，形成广义[淡水螺名称未给出]的亚分支E2（单倍型类群II）。
