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使用仙台病毒载体生成无足迹、高质量的猫诱导多能干细胞。

Generation of footprint-free, high-quality feline induced pluripotent stem cells using Sendai virus vector.

作者信息

Kimura Kazuto, Tsukamoto Masaya, Sugisaki Hiroko, Tanaka Miyuu, Kuwamura Mitsuru, Matsumoto Yuki, Ishihara Genki, Watanabe Kei, Okada Mika, Nakanishi Mahito, Sugiura Kikuya, Hatoya Shingo

机构信息

Department of Advanced Pathobiology, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka 598-8531, Japan.

Department of Integrated Structural Biosciences, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka 598-8531, Japan.

出版信息

Regen Ther. 2024 Sep 2;26:708-716. doi: 10.1016/j.reth.2024.08.012. eCollection 2024 Jun.

DOI:10.1016/j.reth.2024.08.012
PMID:39286639
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11403254/
Abstract

Companion animals, such as felines and canines, could provide an excellent platform for translational research from veterinary to human medicine. However, the use of feline induced pluripotent stems (fiPSCs) of quality in basic or clinical research has not been reported. Here, we generated footprint-free fiPSCs derived from embryonic cells, as well as juvenile feline uterus-derived cells using Sendai virus vector harboring six feline-specific pluripotency-associated genes. The fiPSCs were confirmed to be of high quality with the potential to form teratomas including all three germ layers. Furthermore, our fiPSCs were maintained under feeder-free and chemically-defined conditions using StemFit® AK02N and recombinant laminin 511, iMatrix-511. Further research on fiPSCs could result in their widespread application in veterinary regenerative medicine, which could pave the way for their use in advanced regenerative medicine research for humans.

摘要

伴侣动物，如猫科动物和犬科动物，可为从兽医学到人类医学的转化研究提供一个极好的平台。然而，高质量的猫诱导多能干细胞（fiPSCs）在基础或临床研究中的应用尚未见报道。在此，我们使用携带六个猫特异性多能性相关基因的仙台病毒载体，从胚胎细胞以及幼年猫子宫来源的细胞中生成了无足迹的fiPSCs。这些fiPSCs被证实具有高质量，有形成包含所有三个胚层的畸胎瘤的潜力。此外，我们的fiPSCs使用StemFit® AK02N和重组层粘连蛋白511（iMatrix-511）在无饲养层和化学成分确定的条件下进行培养。对fiPSCs的进一步研究可能会导致它们在兽医再生医学中的广泛应用，这可能为它们在人类先进再生医学研究中的应用铺平道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba69/11403254/00baf63a0b6d/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba69/11403254/b08659a955a5/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba69/11403254/29e494144f44/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba69/11403254/bf0eec3b411a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba69/11403254/d5a6ddd2d1b8/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba69/11403254/00baf63a0b6d/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba69/11403254/b08659a955a5/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba69/11403254/29e494144f44/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba69/11403254/bf0eec3b411a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba69/11403254/d5a6ddd2d1b8/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba69/11403254/00baf63a0b6d/gr4.jpg

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Cell Therapy in Veterinary Medicine as a Proof-of-Concept for Human Therapies: Perspectives From the North American Veterinary Regenerative Medicine Association.
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Front Vet Sci. 2021 Nov 30;8:779109. doi: 10.3389/fvets.2021.779109. eCollection 2021.
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