从人类多能干细胞生成确定性造血祖细胞的方案。
Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells.
机构信息
Institute for Cancer and Genomic Sciences, College of Medicine and Dentistry, University of Birmingham, Birmingham B152TT, UK.
Murdoch Children's Research Institute, The Royal Children's Hospital, Flemington Road, Parkville, VIC 3052, Australia.
出版信息
STAR Protoc. 2020 Oct 16;1(3):100130. doi: 10.1016/j.xpro.2020.100130. eCollection 2020 Dec 18.
This protocol offers a detailed procedure for the differentiation of human pluripotent stem cells (hPSCs) to multipotent hematopoietic progenitors that arise from SOX17 hemogenic endothelium, mimicking intra-embryonic, HOXA-positive, aorta-gonad mesonephros (AGM) hematopoiesis. The generated endothelium displays transcriptional similarities to cells sorted from human 5-week AGM, and CD45CD34RUNX1C progenitors share an accessible chromatin profile with adult hematopoietic stem cells and multipotent progenitors. Therefore, this protocol is suitable for the mechanistic study of human multipotent progenitor development and for modeling childhood leukemias. For complete details on the use and execution of this protocol, please refer to Nafria et al. (2020).
本方案提供了一种详细的程序，用于将人多能干细胞（hPSCs）分化为多能造血祖细胞，这些祖细胞源自 SOX17 血发生内皮细胞，模拟了胚胎内、HOXA 阳性、主动脉-性腺-中肾（AGM）造血。生成的内皮细胞在转录上与从人类 5 周龄 AGM 分选的细胞相似，并且 CD45CD34RUNX1C 祖细胞与成人造血干细胞和多能祖细胞具有可及染色质图谱。因此，该方案适用于人类多能祖细胞发育的机制研究和儿童白血病的建模。有关该方案使用和执行的完整详细信息，请参阅 Nafria 等人（2020 年）。
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