Department of Hand and Foot Surgery, China-Japan Union Hospital of Jilin University, No.126, Xiantai Street, Changchun, 130033, China.
Key Laboratory of Peripheral Nerve Injury and Regeneration of Jilin Province, No.126, Xiantai Street, Changchun, 130033, China.
Adv Sci (Weinh). 2024 Nov;11(41):e2405265. doi: 10.1002/advs.202405265. Epub 2024 Sep 17.
Animal models of peripheral nerve injury (PNI) serve as the fundamental basis for the investigations of nerve injury, regeneration, and neuropathic pain. The injury properties of such models, including the intensity and duration, significantly influence the subsequent pathological changes, pain development, and therapeutic efficacy. However, precise control over the intensity and duration of nerve injury remains challenging within existing animal models, thereby impeding accurate and comparative assessments of relevant cases. Here, a new model that provides quantitative and off-body controllable injury properties via a magnetically controlled clamp, is presented. The clamp can be implanted onto the rat sciatic nerve and exert varying degrees of compression under the control of an external magnetic field. It is demonstrated that this model can accurately simulate various degrees of pathology of human patients by adjusting the magnetic control and reveal specific pathological changes resulting from intensity heterogeneity that are challenging to detect previously. The controllability and quantifiability of this model may significantly reduce the uncertainty of central response and inter-experimenter variability, facilitating precise investigations into nerve injury, regeneration, and pain mechanisms.
动物外周神经损伤（PNI）模型是神经损伤、再生和神经病理性疼痛研究的基础。这些模型的损伤特性，包括强度和持续时间，会显著影响后续的病理变化、疼痛发展和治疗效果。然而，现有的动物模型在精确控制神经损伤的强度和持续时间方面仍具有挑战性，从而阻碍了对相关病例的准确和比较评估。在这里，我们提出了一种新的模型，该模型通过磁控夹具提供定量的、可体外控制的损伤特性。该夹具可以植入大鼠坐骨神经，并在外磁场的控制下施加不同程度的压缩。研究表明，通过调整磁控，该模型可以准确模拟人类患者的各种病理程度，并揭示以前难以检测到的强度异质性引起的特定病理变化。该模型的可控性和可量化性可能会显著降低中枢反应和实验者间变异性的不确定性，有助于精确研究神经损伤、再生和疼痛机制。
