Luo Yongjun, Gao Zhenglan, Guo Honglei, Duan Kunyuan, Lan Tianyu, Tao Buhui, Shen Xiangchun, Guo Qianqian
The State Key Laboratory of Functions and Applications of Medicinal Plants, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang, Guizhou 561113, China.
The Guizhou Provincial Scientific and Technologic Innovation Base ([2023]003), Guizhou Medical University, Guian New District, Guizhou 561113, China.
ACS Appl Mater Interfaces. 2024 Sep 25;16(38):51480-51495. doi: 10.1021/acsami.4c10198. Epub 2024 Sep 17.
The challenge of drug-resistant bacteria-induced wound healing in clinical and public healthcare settings is significant due to the negative impacts on surrounding tissues and difficulties in monitoring the healing progress. We developed photothermal antibacterial nanorods (AuNRs-PU) with the aim of selectively targeting and combating drug-resistant (). The AuNRs-PU were engineered with a bacterial-specific targeting polypeptide (UBI) and a bacterial adhesive carbohydrate polymer composed of galactose and phenylboronic acid. The objective was to facilitate sutureless wound closure by specially distinguishing between bacteria and nontarget cells and subsequently employing photothermal methods to eradicate the bacteria. AuNRs-PU demonstrated high photothermal conversion efficiency in 808 nm laser and effectively caused physical harm to drug-resistant . By integrating the multifunctional bacterial targeting copolymer onto AuNRs, AuNRs-PU showed rapid and efficient bacterial targeting and aggregation in the presence of bacteria and cells, consequently shielding cells from bacterial harm. In a diabetic rat wound model, AuNRs-PU played a crucial role in enhancing healing by markedly decreasing inflammation and expediting epidermis formation, collagen deposition, and neovascularization levels. Consequently, the multifunctional photothermal therapy shows promise in addressing the complexities associated with managing drug-resistant infected wound healing.
在临床和公共卫生保健环境中，耐药菌引起的伤口愈合面临重大挑战，因为其会对周围组织产生负面影响，且难以监测愈合进程。我们开发了光热抗菌纳米棒（AuNRs-PU），旨在选择性地靶向并对抗耐药菌。AuNRs-PU通过细菌特异性靶向多肽（UBI）和由半乳糖与苯硼酸组成的细菌粘附性碳水化合物聚合物进行工程设计。目的是通过特别区分细菌和非靶细胞，随后采用光热方法根除细菌，从而促进无缝合伤口闭合。AuNRs-PU在808 nm激光下表现出高光热转换效率，并有效对耐药菌造成物理损伤。通过将多功能细菌靶向共聚物整合到AuNRs上，AuNRs-PU在有细菌和细胞存在的情况下显示出快速有效的细菌靶向和聚集，从而保护细胞免受细菌伤害。在糖尿病大鼠伤口模型中，AuNRs-PU通过显著减轻炎症并加速表皮形成、胶原蛋白沉积和新血管形成水平，在促进愈合方面发挥了关键作用。因此，多功能光热疗法在解决与耐药菌感染伤口愈合管理相关的复杂性方面显示出前景。
