Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, People's Republic of China.
Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, People's Republic of China.
ACS Nano. 2023 Nov 14;17(21):22106-22120. doi: 10.1021/acsnano.3c09220. Epub 2023 Oct 30.
Diabetic wounds represent a persistent global health challenge with a substantial impact on patients' health and overall well-being. Herein, a hydrogel system that integrates functionalized gold nanorods (AuNRs) and M2 macrophage-derived exosomes (M2-Exos) was developed to achieve an efficient and synergistic therapy for diabetic wounds. We introduced an ion-cross-linked dissipative network into a prefabricated covalent cross-linked network (long-chain polymer network), which was prepared using AuNRs as a specific cross-linker. The ion network was then cross-linked with the long-chain polymer to form a specific network structure, imparting antiswelling and photothermal effects to the hydrogel. This integrated hydrogel system effectively scavenged reactive oxygen species levels, inhibited inflammation, promoted angiogenesis, and stimulated photothermal antibacterial activity through near-infrared (NIR) irradiation. To demonstrate the potential of the hydrogel, we established experimental animal models of oral mucosa ulceration and full-thickness skin defects. results confirmed that M2-Exos released from the hydrogels played a crucial role in wound closure. Furthermore, the synergistic effect of AuNRs and NIR photothermal effects eradicated bacterial infections in the wound area. Overall, our integrated hydrogel system is a promising tool for accelerating chronic diabetic wound healing and tissue regeneration. This study highlights the potential benefits of combining bioactive M2-Exos and the photothermal effect of AuNRs into an antiswelling hydrogel platform to achieve satisfactory wound healing in patients with diabetes.
糖尿病创面是一个全球性的医学难题，对患者的健康和整体幸福感有重大影响。本研究开发了一种水凝胶系统，将功能化金纳米棒（AuNRs）和 M2 巨噬细胞衍生的外泌体（M2-Exos）集成在一起，实现了糖尿病创面的高效协同治疗。我们将离子交联耗散网络引入预制的共价交联网络（长链聚合物网络）中，该网络使用 AuNRs 作为特定交联剂制备。然后，离子网络与长链聚合物交联，形成特定的网络结构，赋予水凝胶抗溶胀和光热效应。该集成水凝胶系统通过近红外（NIR）照射有效地清除活性氧水平、抑制炎症、促进血管生成，并刺激光热抗菌活性。为了证明水凝胶的潜力，我们建立了口腔黏膜溃疡和全层皮肤缺损的实验动物模型。结果证实，水凝胶中释放的 M2-Exos 在创面闭合中起着关键作用。此外，AuNRs 和 NIR 光热效应的协同作用消除了创面区域的细菌感染。总之，我们的集成水凝胶系统是加速慢性糖尿病创面愈合和组织再生的有前途的工具。本研究强调了将生物活性 M2-Exos 和 AuNRs 的光热效应结合到抗溶胀水凝胶平台中以实现糖尿病患者满意的创面愈合的潜力。
