Department of Allied Health Sciences, Brainware University, 398, Ramkrishnapur Road, Kolkata, West Bengal 700125, India.
Department of Polymer Science & Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India.
ACS Appl Bio Mater. 2024 Oct 21;7(10):6414-6429. doi: 10.1021/acsabm.4c00551. Epub 2024 Sep 17.
The intricate healing mechanism of chronic wounds and their multitude of healing-related obstacles, such as infections, compromised cellular processes, and impediments to the healing process, pose a significant healthcare problem. Exploration of metal oxide nanoparticles, such as yttrium oxide (YO) nanoparticles, can lead to innovative discoveries in the field of chronic wound healing by offering cues that promote cell proliferation in the scaffolds. To achieve this, YO nanoparticles were synthesized and incorporated within poly(vinyl alcohol) (PVA) nanofibrous scaffolds. Moreover, lysine was infused in the nanofibrous scaffolds to tune its cell adhesion and antimicrobial property. The structure and morphology of the synthesized nanofibers were confirmed through various physicochemical characterizations. Notably, all the fabricated scaffolds have remarkably tuned WVTR values within the range of 2000-2500 g/m/day, favorable for removing the wound exudate, which facilitate the healing process. The scaffolds exhibited substantial antimicrobial property of approximately 68% and 72.2% against both and at optimized YO loading. They further prevented the formation of biofilm by 68.6% for and 51.2% for , suggesting the inhibition of recurrent wound infection. The scaffolds illustrated good blood biocompatibility, cytocompatibility, and cell adhesion capabilities. In vitro ROS inhibition study also corroborated the antioxidant property of the scaffold. Similarly, the wound scratching experiment showed high proliferative capability of a yttria-loaded PVA/lysine (S3) sample through the development of an extracellular matrix support. Molecular insight of wound healing was also validated through flow cytometry analysis and immunocytochemistry imaging studies. The findings revealed increased collagen I (Col-I) expression of approximately 19.48% in cultured fibrocytes. The findings are validated from immunocytochemistry imaging. In summary, the results furnish a captivating paradigm for the use of these scaffolds as a therapeutic biomaterial and to foster their potential efficacy toward wound care management.
慢性伤口的复杂愈合机制及其众多与愈合相关的障碍，如感染、细胞过程受损以及愈合过程受阻，构成了重大的医疗保健问题。探索金属氧化物纳米粒子，如氧化钇（YO）纳米粒子，可以通过提供促进支架中细胞增殖的线索，为慢性伤口愈合领域带来创新发现。为了实现这一目标，合成了 YO 纳米粒子并将其掺入聚（乙烯醇）（PVA）纳米纤维支架中。此外，赖氨酸被注入纳米纤维支架中以调节其细胞粘附和抗菌性能。通过各种物理化学特性分析证实了合成纤维的结构和形态。值得注意的是，所有制备的支架的 WVTR 值都在 2000-2500 g/m/天的范围内得到了很好的调节，有利于去除伤口渗出物，从而促进愈合过程。支架在优化的 YO 负载下对 和 均表现出约 68%和 72.2%的显著抗菌性能。它们还通过 68.6%的 和 51.2%的 防止生物膜的形成，表明抑制了复发性伤口感染。支架显示出良好的血液生物相容性、细胞相容性和细胞粘附能力。体外 ROS 抑制研究也证实了支架的抗氧化特性。同样，划痕实验表明，负载氧化钇的 PVA/赖氨酸（S3）样品通过开发细胞外基质支持具有很高的增殖能力。通过流式细胞术分析和免疫细胞化学成像研究也验证了伤口愈合的分子机制。结果表明，培养的成纤维细胞中胶原蛋白 I（Col-I）的表达增加了约 19.48%。免疫细胞化学成像验证了这些发现。总之，这些结果为这些支架作为治疗性生物材料的应用提供了一个引人入胜的范例，并促进了它们在伤口护理管理方面的潜在疗效。
