Key Laboratory of Science and Technology of Eco-Textile & Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, Donghua University, Shanghai 201620, P. R. China.
Co-Innovation Center of Efficient Processing and Utilization of Forestry Resources, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, Jiangsu 210037, P. R. China.
J Mater Chem B. 2021 May 5;9(17):3727-3744. doi: 10.1039/d1tb00217a.
Bacterial infections cause severe secondary damage to wounds and hinder wound healing processes. We prepared magnesium oxide (MgO) nanoparticle-incorporated nanofibrous membranes by electrospinning and investigated their potential for wound dressing and fighting bacterial infection. MgO-Incorporated membranes possessed good elasticity and flexibility similar to native skin tissue and were hydrophilic, ensuring comfortable contact with wound beds. The cytocompatibility of membranes was dependent on the amounts of incorporated MgO nanoparticles: lower amounts promoted while higher amounts suppressed the proliferation of fibroblasts, endothelial cells, and macrophages. The antibacterial capacity of membranes was proportional to the amounts of incorporated MgO nanoparticles and they inhibited more than 98% E. coli, 90% S. aureus, and 94% S. epidermidis. MgO nanoparticle-incorporated membranes effectively suppressed bacterial infection and significantly promoted the healing processes of infected full-thickness wounds in a rat model. Subcutaneous implantation demonstrated that the incorporation of MgO nanoparticles into electrospun membranes elevated their bioactivity as evidenced by considerable cell infiltration into their dense nanofiber configuration and enhanced the remodeling of implanted membranes. This study highlights the potential of MgO-incorporated electrospun membranes in preventing bacterial infections of wounds.
细菌感染会对伤口造成严重的继发性损伤，并阻碍伤口愈合过程。我们通过静电纺丝制备了氧化镁 (MgO) 纳米粒子复合纳米纤维膜，并研究了其在伤口敷料和抗菌感染方面的潜力。MgO 复合膜具有与天然皮肤组织相似的良好弹性和柔韧性，且具有亲水性，可确保与伤口床舒适接触。膜的细胞相容性取决于复合的 MgO 纳米粒子的量：较低的量促进而较高的量抑制成纤维细胞、内皮细胞和巨噬细胞的增殖。膜的抗菌能力与复合的 MgO 纳米粒子的量成正比，它们抑制了超过 98%的大肠杆菌、90%的金黄色葡萄球菌和 94%的表皮葡萄球菌。MgO 纳米粒子复合膜有效抑制了细菌感染，并显著促进了大鼠模型中感染性全层伤口的愈合过程。皮下植入表明，将 MgO 纳米粒子掺入电纺膜中提高了其生物活性，大量细胞渗透到其密集的纳米纤维结构中，并增强了植入膜的重塑。这项研究强调了 MgO 复合电纺膜在预防伤口细菌感染方面的潜力。
