Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, PR China; Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China; College of Food Science, Northeast Agricultural University, Harbin 150030, PR China.
Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, PR China.
Food Chem. 2025 Jan 15;463(Pt 2):141228. doi: 10.1016/j.foodchem.2024.141228. Epub 2024 Sep 10.
A three-mode biosensor incorporated ratiometric (electrochemical/colorimetric, electrochemical/photothermal) into its design was constructed using DNA-driven magnetic beads (DMBs) as a bridge to detect C. perfringens. It further enhances the accuracy of detection results while maintaining compatibility with applications in multiple scenarios. Briefly, the G-quadruplex was combined with aptamer and immobilized onto magnetic beads through amide-bond, resulting in the integration of DMBs. The DMBs and supernatant were separated by magnetic separation when the target was present. Subsequently, the DMBs were utilized to construct the electrochemical biosensor, whereas the supernatant was used to construct colorimetric and photothermal biosensors. The limits of detection the ratiometric biosensor were ultimately reduced to 0.26 and 0.27 lg CFU g, respectively, in comparison to the single three-mode biosensor. Moreover, this biosensor had been applied in real-sample assays successfully. The establishment of this platform provides a new method for detecting pathogens in the fields of food safety and environmental monitoring.
构建了一种三模式生物传感器，将比率（电化学/比色法、电化学/光热）设计纳入其中，使用 DNA 驱动的磁性珠（DMB）作为桥梁来检测产气荚膜梭菌。它在保持与多种场景应用兼容性的同时，进一步提高了检测结果的准确性。简要地说，G-四链体与适体结合，并通过酰胺键固定在磁性珠上，从而形成 DMB 的整合。当存在目标时，通过磁分离将 DMB 和上清液分离。随后，DMB 用于构建电化学生物传感器，而上清液用于构建比色法和光热生物传感器。与单一的三模式生物传感器相比，比率生物传感器的检测限最终降低到 0.26 和 0.27 lg CFU g。此外，该生物传感器已成功应用于实际样品检测。该平台的建立为食品安全和环境监测领域的病原体检测提供了一种新方法。
