Guo Shanshan, Zhang Qi, Li Xiaoxiao, Wang Qiaozhi, Li Xia, Wang Pin, Xue Qingwang
School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong, China.
School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong, China.
Colloids Surf B Biointerfaces. 2025 Jan;245:114213. doi: 10.1016/j.colsurfb.2024.114213. Epub 2024 Sep 6.
Skin wounds are susceptible to bacterial infections, which hinder healing and extend recovery. Herein, we designed a silver nanoclusters (Ag NCs) composite hydrogel for infected wound treatment via bacterial enzymatic degradation and Ag release. Using biocompatible gelatine and polyethylene glycol as the main components, DNA-templated Ag NCs were covalently linked to a polymer network to obtain the final nanocomposite hydrogel. This hydrogel exhibited good compressive and tensile stiffness, bioadhesion and water absorption. The overexpressed bacterial enzymes protease and DNase in the infected wound were hydrolysed by the gel matrix, subsequently releasing antibacterial Ag ions. In vitro experimental results proved that the hydrogel demonstrated excellent bactericidal effect on Staphylococcus aureus and Escherichia coli, which are commonly implicated in clinical wound infections. Animal experiments revealed that the hydrogel considerably promoted cell proliferation and wound healing with less inflammatory responses. Thus, these results demonstrate strategies for bacterial enzyme-responsive Ag release for infected wound healing, facilitating further development of intelligent bandages.
皮肤伤口易受细菌感染，这会阻碍伤口愈合并延长恢复时间。在此，我们设计了一种银纳米簇（Ag NCs）复合水凝胶，用于通过细菌酶降解和银释放来治疗感染伤口。以生物相容性明胶和聚乙二醇为主要成分，将DNA模板化的Ag NCs共价连接到聚合物网络上，从而获得最终的纳米复合水凝胶。这种水凝胶表现出良好的压缩和拉伸刚度、生物粘附性和吸水性。感染伤口中过表达的细菌酶蛋白酶和脱氧核糖核酸酶被凝胶基质水解，随后释放出抗菌银离子。体外实验结果证明，该水凝胶对临床伤口感染中常见的金黄色葡萄球菌和大肠杆菌具有优异的杀菌效果。动物实验表明，该水凝胶能显著促进细胞增殖和伤口愈合，且炎症反应较少。因此，这些结果展示了用于感染伤口愈合的细菌酶响应性银释放策略，有助于智能绷带的进一步发展。
