He Yingjie, Chen Jing, Xu Zhao, Nie Junqi, Wang Feiyi, Ma Chao, Wang Cheng, Zhang Liang, Lu Cuifen
Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, China.
Department of Dermatology, Traditional Chinese and Western Medicine Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Department of Dermatology, Wuhan No.1 Hospital, Wuhan 430022, China.
Int J Biol Macromol. 2025 Jan;285:138290. doi: 10.1016/j.ijbiomac.2024.138290. Epub 2024 Dec 2.
Bacterial infections during wound healing often cause inflammation, which delays the healing process. Therefore, innovative wound dressings are urgently needed to inhibit bacterial infections and promote healing. This study proposes an Ag-functionalized chitosan hydrogel dressing, formed via a Schiff-base reaction between alkynyl Ag substituted chitosan (Ag-CS) and octafunctionalized polyhedral oligomeric silsesquioxane with benzaldehyde-terminated polyethylene glycol (POSS-PEG-CHO), to address the issue of bacterial infection in wounds. The hydrogel demonstrated excellent injectability and self-healing properties. AgNPs and alkynyl Ag, produced by the reducing effect of chitosan and the reversible reaction of alkynyl Ag, delay the release of Ag. Furthermore, the hydrogel exhibits a broad-spectrum antibacterial effect and effectively inhibits bacterial biofilm formation. The release of Ag lasts for 7 days, ensuring sustainable antibacterial properties. In a mouse infected wound model, the composite hydrogel significantly accelerated wound healing. By the eighth day, the wound healing rate reached 99 %, whereas the control group achieved only 91 %. Histological and immunofluorescence staining results indicated that hydrogel-treated wounds had faster re-epithelialization, collagen deposition, and angiogenesis, with reduced inflammation. In conclusion, the Ag-functionalized chitosan hydrogel, with sustained Ag release and enhanced antibacterial properties, shows great potential as a wound dressing for promoting the healing of infected wounds.
伤口愈合期间的细菌感染通常会引发炎症，从而延缓愈合进程。因此，迫切需要创新型伤口敷料来抑制细菌感染并促进愈合。本研究提出一种银功能化壳聚糖水凝胶敷料，它是通过炔基银取代壳聚糖（Ag-CS）与带有苯甲醛封端聚乙二醇的八官能化多面体低聚倍半硅氧烷（POSS-PEG-CHO）之间的席夫碱反应形成的，以解决伤口细菌感染问题。该水凝胶表现出优异的可注射性和自愈性能。壳聚糖的还原作用以及炔基银的可逆反应产生的AgNPs和炔基银，延缓了银的释放。此外，该水凝胶具有广谱抗菌作用，并能有效抑制细菌生物膜形成。银的释放持续7天，确保了持续的抗菌性能。在小鼠感染伤口模型中，复合水凝胶显著加速了伤口愈合。到第8天时，伤口愈合率达到99%，而对照组仅为91%。组织学和免疫荧光染色结果表明，经水凝胶处理的伤口上皮再形成、胶原蛋白沉积和血管生成更快，炎症减轻。总之，具有持续银释放和增强抗菌性能的银功能化壳聚糖水凝胶，作为促进感染伤口愈合的伤口敷料具有巨大潜力。
